Filamentous fungi have been of great interest because of their excellent ability as cell
factories to manufacture useful products for human beings. The development of genetic …

Molecular genetic manipulation of micro‐organisms requires the development of plasmid‐
mediated transformation systems that include:(i) infusion of exogenous DNA into recipient …

The production of transgenic fungi is a routine process. Currently, it is possible to insert
genes from other fungi, viruses, bacteria and even animals, albeit with low efficiency, into the …

Genetic transformation is an essential tool for the modern study of gene function and the
genetic improvement of an organism. The genetic transformation of many fungal species is …

Filamentous fungi have a long-standing tradition as industrial producers of primary and
secondary metabolites. Initially, industrial scientists selected production strains from natural …

Recombinant DNA technology offers one the potential to make well-defined alterations in
the genetic make-up of an organism. The development of this technology was based on two …

The protoplast method for the transformation of filamentous fungi is described in detail, as is
the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged …

Advances in genetic transformation techniques have made important contributions to
molecular genetics. Various molecular tools and strategies have been developed for …

Plant diseases caused by plant pathogenic fungi continuously threaten the sustainability of
global crop production. An effective way to study the disease-causing mechanisms of these …

Agrobacterium tumefaciens transfers part of its Ti plasmid, the T-DNA, to plant cells during
tumorigenesis. It is routinely used for the genetic modification of a wide range of plant …