Engineering of Pseudomonas putida for accelerated co-utilization of glucose and cellobiose yields aerobic overproduction of pyruvate explained by an upgraded …

D Bujdoš, B Popelářová, DC Volke, PI Nikel… - Metabolic …, 2023 - Elsevier
Pseudomonas putida KT2440 is an attractive bacterial host for biotechnological production
of valuable chemicals from renewable lignocellulosic feedstocks as it can valorize lignin …

[HTML][HTML] Refactoring the upper sugar metabolism of Pseudomonas putida for co-utilization of cellobiose, xylose, and glucose

P Dvořák, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
Given its capacity to tolerate stress, NAD (P) H/NAD (P) balance, and increased ATP levels,
the platform strain Pseudomonas putida EM42, a genome-edited derivative of the soil …

Functional implementation of a linear glycolysis for sugar catabolism in Pseudomonas putida

A Sánchez-Pascuala, L Fernández-Cabezón… - Metabolic …, 2019 - Elsevier
The core metabolism for glucose assimilation of the soil bacterium and platform strain
Pseudomonas putida KT2440 has been reshaped from the native, cyclically-operating …

Growth of engineered Pseudomonas putida KT2440 on glucose, xylose, and arabinose: Hemicellulose hydrolysates and their major sugars as sustainable carbon …

Y Wang, F Horlamus, M Henkel, F Kovacic… - GCB …, 2019 - Wiley Online Library
Lignocellulosic biomass is the most abundant bioresource on earth containing polymers
mainly consisting of d‐glucose, d‐xylose, l‐arabinose, and further sugars. In order to …

Engineered Pseudomonas putida KT2440 co-utilizes galactose and glucose

GL Peabody, JR Elmore, J Martinez-Baird… - Biotechnology for …, 2019 - Springer
Background Efficient conversion of plant biomass to commodity chemicals is an important
challenge that needs to be solved to enable a sustainable bioeconomy. Deconstruction of …

Engineering Pseudomonas putida S12 for Efficient Utilization of d-Xylose and l-Arabinose

JP Meijnen, JH de Winde… - Applied and …, 2008 - Am Soc Microbiol
The solvent-tolerant bacterium Pseudomonas putida S12 was engineered to utilize xylose
as a substrate by expressing xylose isomerase (XylA) and xylulokinase (XylB) from …

Reconciling in vivo and in silico key biological parameters of Pseudomonas putidaKT2440 during growth on glucose under carbon-limited condition

JBJH van Duuren, J Puchałka, AE Mars, R Bücker… - BMC …, 2013 - Springer
Background Genome scale metabolic reconstructions are developed to efficiently engineer
biocatalysts and bioprocesses based on a rational approach. However, in most …

Engineered Pseudomonas putida simultaneously catabolizes five major components of corn stover lignocellulose: Glucose, xylose, arabinose, p-coumaric acid, and …

JR Elmore, GN Dexter, D Salvachúa, M O'Brien… - Metabolic …, 2020 - Elsevier
Valorization of all major lignocellulose components, including lignin, cellulose, and
hemicellulose is critical for an economically viable bioeconomy. In most biochemical …

Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio‐based economy

F Horlamus, Y Wang, D Steinbach… - Gcb …, 2019 - Wiley Online Library
Lignocellulose‐derived hydrolyzates typically display a high degree of variation depending
on applied biomass source material as well as process conditions. Consequently, this …

Metabolic engineering of Propionibacterium freudenreichii subsp. shermanii for xylose fermentation

P Wei, M Lin, Z Wang, H Fu, H Yang, W Jiang… - Bioresource …, 2016 - Elsevier
Propionibacterium freudenreichii cannot use xylose, the second most abundant sugar in
lignocellulosic biomass. Although Propionibacterium acidipropionici can use xylose as a …