Hydrogen peroxide and nitric oxide are involved in programmed cell death induced by cryopreservation in Dendrobium protocorm-like bodies

X Jiang, R Ren, W Di, M Jia, Z Li, Y Liu… - Plant Cell, Tissue and …, 2019 - Springer
Programmed cell death (PCD) plays a key role in animal tissue cell death following
cryopreservation. However, there are few studies evaluating the plant PCD characteristics …

Exogenous ethylene increases the viability of cryopreserved Dendrobium protocorm-like bodies by regulating the hydrogen peroxide and its mediated oxidative …

L Zhang, R Ren, X Jiang, H Zhou, Y Liu - Plant Cell, Tissue and Organ …, 2021 - Springer
Cryopreservation, an effective method for long-term preservation of germplasm resources,
but after liquid nitrogen (LN) preservation, the survival rate of some species/cultivars has …

Exogenous catalase and pyruvate dehydrogenase improve survival and regeneration and affect oxidative stress in cryopreserved Dendrobium nobile protocorm-like …

W Di, M Jia, J Xu, B Li, Y Liu - CryoLetters, 2017 - ingentaconnect.com
BACKGROUND: Reactive oxygen species (ROS)-induced oxidative damage is responsible
for viability loss in plant tissues following cryopreservation. Antioxidants may improve …

ROS-induced oxidative stress in nobile-type Dendrobium protocorm-like bodies (PLBs) during vitrification

M Jia, W Di, Y Liu, Y Shi, Y Xie - Cryoletters, 2016 - ingentaconnect.com
BACKGROUND: Oxidative stress involved in cryopreservation protocols may be responsible
for the poor survival of tissues after cryopreservation. OBJECTIVE: In the current study, we …

Stress and damage mechanisms in Dendrobium nobile Lindl. protocorm-like bodies during pre- and post-liquid nitrogen exposure in cryopreservation revealed by …

W Di, X Jiang, J Xu, M Jia, B Li, Y Liu - In Vitro Cellular & Developmental …, 2018 - Springer
Liquid nitrogen (LN) exposure, a crucial step in cryopreservation, is mainly responsible for
the reduced viability of protocorm-like bodies (PLBs) in Dendrobium nobile Lindl. To …

ROS-induced oxidative stress and apoptosis-like event directly affect the cell viability of cryopreserved embryogenic callus in Agapanthus praecox

D Zhang, L Ren, G Chen, J Zhang, BM Reed, X Shen - Plant cell reports, 2015 - Springer
Key message Oxidative stress and apoptosis-like programmed cell death, induced in part by
H 2 O 2, are two key factors that damage cells during plant cryopreservation. Their inhibition …

Cryopreservation of Dendrobium candidum Wall. ex Lindl. protocorm-like bodies by encapsulation-vitrification

M Yin, S Hong - Plant Cell, Tissue and Organ Culture (PCTOC), 2009 - Springer
Protocorm-like bodies (PLBs) of Dendrobium candidum Wall. ex Lindl., orchid, were
successfully cryopreserved using an encapsulation vitrification method. PLBs were …

Encapsulation-vitrification of Dendrobium sonia-28 supported by histology

LP Ching, JJJ Antony, R Poobathy… - Plant Omics, 2012 - search.informit.org
In vitro protocorm-like bodies (PLBs) of Dendrobium sonia-28 were cryopreserved through
an encapsulation-vitrification method. One to two and 3-4mm PLBs were precultured in half …

Phloroglucinol enhances recovery and survival of cryopreserved Dendrobium nobile protocorms

WA Vendrame, RT Faria - Scientia Horticulturae, 2011 - Elsevier
The aim of the present study was to evaluate the effect of phloroglucinol in the recovery and
survival of cryopreserved Dendrobium nobile protocorms. The exposure of protocorms to 2M …

Effects of Ascorbic Acid on PVS2 Cryopreservation of Dendrobium Bobby Messina's PLBs Supported with SEM Analysis

JJJ Antony, CL Keng, M Mahmood… - Applied biochemistry and …, 2013 - Springer
Regrowth of the cryopreserved protocorm-like bodies (PLBs) of Dendrobium Bobby Messina
was assessed based on the plant vitrification solution 2 (PVS2) optimisation conditions. The …