Continuous quadrupole magnetic separation of islets during digestion improves purified porcine islet viability
BP Weegman, VS Kumar Sajja… - Journal of Diabetes …, 2016 - Wiley Online Library
Islet transplantation (ITx) is an emerging and promising therapy for patients with
uncontrolled type 1 diabetes. The islet isolation and purification processes require exposure …
uncontrolled type 1 diabetes. The islet isolation and purification processes require exposure …
Quadrupole magnetic sorting of porcine islets of Langerhans
RM Shenkman, JJ Chalmers, BJ Hering… - … Engineering Part C …, 2009 - liebertpub.com
Islet transplantation is emerging as a treatment option for selected patients with type 1
diabetes. Inconsistent isolation, purification, and recovery of large numbers of high-quality …
diabetes. Inconsistent isolation, purification, and recovery of large numbers of high-quality …
Surgical protocol involving the infusion of paramagnetic microparticles for preferential incorporation within porcine islets
MD Rizzari, TM Suszynski, LS Kidder, SA Stein… - Transplantation …, 2010 - Elsevier
INTRODUCTION: Despite significant advances, widespread applicability of islet cell
transplantation remains elusive. Refinement of current islet isolation protocols may improve …
transplantation remains elusive. Refinement of current islet isolation protocols may improve …
Immunomagnetic isolation of islets from the rat pancreas
P Nandigala, TH Chen, C Yang, WH Hsu… - Biotechnology …, 1997 - Wiley Online Library
Islets were selectively isolated from rat pancreatic digests using magnetic microspheres
coated with anti‐islet monoclonal antibodies. The isolation process was optimized as a …
coated with anti‐islet monoclonal antibodies. The isolation process was optimized as a …
Optimization of the magnetic field used for immunomagnetic islet purification
JE Davies, RFL James, NJM London… - …, 1995 - journals.lww.com
Purification of islets based on the physical differences in density between exocrine and islet
tissue reduces islet yields and remains one of the factors limiting islet transplantation …
tissue reduces islet yields and remains one of the factors limiting islet transplantation …
A COMPARISON OF THE USE OF TWO IMMUNOMAGNETIC MICROSPHERES FOR SECONDARY PURIFICATION OF PANCREATIC ISLETS1
JE Davies, S Winoto-Morbach, K Ulrichs… - …, 1996 - journals.lww.com
Immunomagnetic cell separation has been shown to be a highly attractive alternative to
density-dependent methods for islet purification. There are two types of beads, magnetic …
density-dependent methods for islet purification. There are two types of beads, magnetic …
New principle for large-scale preparation of purified human pancreas islets
S Winoto-Morbach, K Ulrichs, G Leyhausen… - Diabetes, 1989 - Am Diabetes Assoc
Because successful human islet transplantation requires large quantities of viable islets that
must be separated from the highly immunogenic exocrine tissue and because handpicking …
must be separated from the highly immunogenic exocrine tissue and because handpicking …
[HTML][HTML] Magnetic particle imaging of islet transplantation in the liver and under the kidney capsule in mouse models
Background Islet transplantation (Tx) represents the most promising therapy to restore
normoglycemia in type 1 diabetes (T1D) patients to date. As significant islet loss has been …
normoglycemia in type 1 diabetes (T1D) patients to date. As significant islet loss has been …
Human islet separation utilizing a closed automated purification system
AS Friberg, M Ståhle, H Brandhorst… - Cell …, 2008 - journals.sagepub.com
A central step within the human islet isolation process is the separation of islets from
contaminating exocrine tissue utilizing linear, continuous density gradients manufactured by …
contaminating exocrine tissue utilizing linear, continuous density gradients manufactured by …
Application of magnetic particle tracking velocimetry to quadrupole magnetic sorting of porcine pancreatic islets
VSK Sajja, TR Hanley, H Gapsis… - Biotechnology and …, 2011 - Wiley Online Library
Magnetic isolation is a promising method for separating and concentrating pancreatic islets
of Langerhans for transplantation in Type 1 diabetes patients. We are developing a …
of Langerhans for transplantation in Type 1 diabetes patients. We are developing a …