Current and future methods for mRNA analysis: a drive toward single molecule sequencing

A Bayega, S Fahiminiya, S Oikonomopoulos… - … Analysis: Methods and …, 2018 - Springer
The transcriptome encompasses a range of species including messenger RNA, and other
noncoding RNA such as rRNA, tRNA, and short and long noncoding RNAs. Due to the huge …

RNA sequencing and analysis

KR Kukurba, SB Montgomery - Cold Spring Harbor Protocols, 2015 - cshprotocols.cshlp.org
RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods
to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing …

SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression

T Nakamura, Y Yabuta, I Okamoto… - Nucleic acids …, 2015 - academic.oup.com
Single-cell mRNA sequencing (RNA-seq) methods have undergone rapid development in
recent years, and transcriptome analysis of relevant cell populations at single-cell resolution …

Bioinformatics for RNA-seq data analysis

S Zhao, B Zhang, Y Zhang, W Gordon… - Bioinformatics …, 2016 - books.google.com
While RNA sequencing (RNA-seq) has become increasingly popular for transcriptome
profiling, the analysis of the massive amount of data generated by large-scale RNA-seq still …

RNA sequencing: advances, challenges and opportunities

F Ozsolak, PM Milos - Nature reviews genetics, 2011 - nature.com
In the few years since its initial application, massively parallel cDNA sequencing, or RNA-
seq, has allowed many advances in the characterization and quantification of …

RNA sequencing: from sample preparation to analysis

S Maekawa, A Suzuki, S Sugano, Y Suzuki - … Factor Regulatory Networks …, 2014 - Springer
The introduction of RNA sequencing (RNA-seq), as a direct result of rapid progression of
next-generation sequencing technologies, has revolutionized the world of transcriptomics. It …

Revealing the history and mystery of RNA-Seq

A Gondane, HM Itkonen - Current Issues in Molecular Biology, 2023 - mdpi.com
Advances in RNA-sequencing technologies have led to the development of intriguing
experimental setups, a massive accumulation of data, and high demand for tools to analyze …

Advancing RNA-seq analysis

BJ Haas, MC Zody - Nature biotechnology, 2010 - nature.com
Advancing RNA-Seq analysis | Nature Biotechnology Skip to main content Thank you for
visiting nature.com. You are using a browser version with limited support for CSS. To obtain the …

Methods, challenges and potentials of single cell RNA-seq

D Hebenstreit - Biology, 2012 - mdpi.com
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several
recent studies demonstrate its successful adaption to single cell analysis. This allows new …

Alternative applications for distinct RNA sequencing strategies

L Han, KC Vickers, DC Samuels… - Briefings in …, 2015 - academic.oup.com
Recent advances in RNA library preparation methods, platform accessibility and cost
efficiency have allowed high-throughput RNA sequencing (RNAseq) to replace conventional …