Background With the availability of genome sequences, gene expression analysis of jute
has drawn considerable attention for understanding the regulatory mechanisms of fiber …

To accurately measure gene expression using quantitative reverse transcription PCR (qRT-
PCR), reliable reference gene (s) are required for data normalization. Corchorus capsularis …

Quantitative real-time PCR (RT-qPCR) has become an accurate and widely used technique
to analyze expression levels of selected genes. It is very necessary to select appropriate …

RT-qPCR is a widely used method in gene expression and transcriptome studies.
Normalization based on reference genes is necessary to accurately analyze RT-qPCR data …

Background Usually the reference genes used in gene expression analysis have been
chosen for their known or suspected housekeeping roles, however the variation observed in …

Lycoris aurea (L'Hér.) Herb, a perennial grass species, produces a unique variety of
pharmacologically active Amaryllidaceae alkaloids. However, the key enzymes and their …

Reliable reference selection for the accurate quantification of gene expression under
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …

Peucedanum praeruptorum Dunn is one of the main traditional Chinese medicines
producing coumarins and plenty of literatures are focused on the biosynthesis of coumarins …

The use of reverse transcription quantitative PCR technology to assess gene expression
levels requires an accurate normalization of data in order to avoid misinterpretation of …

Selecting suitable internal reference genes is an important prerequisite for the application of
quantitative real-time PCR (qRT-PCR). However, no systematic studies have been …