We present a general strategy for determining the cholesterol-binding site of eukaryotic
membrane proteins in native-like lipid membranes by NMR spectroscopy. The strategy …

Experimental evidence for a direct role of lipids in determining the structure, dynamics, and
function of membrane proteins leads to the term 'functional lipids'. In particular, the sterol …

Highlights•Novel solid-state NMR distance techniques have been developed to determine
cholesterol-binding sites in membrane proteins.•Solid-state NMR experiments yield the …

The orientation of lipid headgroups may serve as a powerful sensor of electrostatic
interactions in membranes. As shown previously by 2 H NMR measurements, the …

Cholesterol is a ubiquitous component of mammalian cell membranes and affects
membrane protein function. Although cholesterol-mediated formation of ordered membrane …

Cholesterol is a crucial component of biological membranes and can interact with other
membrane components through hydrogen bonding. NMR spectroscopy has been used …

Investigations of lipid membranes using NMR spectroscopy generally require isotopic
labeling, often precluding structural studies of complex lipid systems. Solid-state 13 C magic …

Cholesterol is a major component of mammalian cell membranes. In erythrocytes and in
myelin, it is present in concentrations that approach those of phospholipids. 1-3 Despite …

Solid-state NMR spectroscopy has been developed for the investigation of membrane-
associated polypeptides and remains one of the few techniques to reveal high-resolution …

While an increasing number of structural biology studies successfully demonstrate the
power of high-resolution structures and dynamics of membrane proteins in fully …