microRNAs as reference genes for quantitative PCR in cotton

AKS Fausto, TF Silva, E Romanel, MFS Vaslin - PLoS One, 2017 - journals.plos.org
Cotton (Gossypium hirsutum) is the most important non-food plant in the world. Studies
concerning the fiber quality and plant fitness of cotton at molecular level depend on high …

Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar

F Tang, L Chu, W Shu, X He, L Wang, M Lu - Plant Methods, 2019 - Springer
Background Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is a
rapid and sensitive approach to identify miRNA and protein-coding gene expression in …

Expression of microRNAs in cotton

B Zhang, X Pan - Molecular biotechnology, 2009 - Springer
MicroRNAs (miRNAs) are a class of newly identified non-protein-coding small RNAs.
miRNAs post-transcriptionally regulate the expression of more than 30% genes, which …

Selection of candidate reference genes for real-time PCR studies in lettuce under abiotic stresses

JM Borowski, V Galli, R da Silva Messias, EC Perin… - Planta, 2014 - Springer
The process of selection and validation of reference genes is the first step in studies of gene
expression by real-time quantitative polymerase chain reaction (RT-qPCR). The genome of …

Selection of suitable inner reference genes for relative quantification expression of microRNA in wheat

H Feng, X Huang, Q Zhang, G Wei, X Wang… - Plant Physiology and …, 2012 - Elsevier
Quantitative real-time PCR (qRT-PCR) is currently the most accurate and widely applied
method to detect differential genes expression, but choosing a suitable gene to be the …

Identification and Characterization of miRNA Transcriptome in Asiatic Cotton (Gossypium arboreum) Using High Throughput Sequencing

M Farooq, S Mansoor, H Guo, I Amin… - Frontiers in Plant …, 2017 - frontiersin.org
MicroRNAs (miRNAs) are small 20–24nt molecules that have been well studied over the
past decade due to their important regulatory roles in different cellular processes. The …

Evaluation of appropriate reference gene for normalization of microRNA expression by real-time PCR in Lablab purpureus under abiotic stress conditions

A Thilagavathy, VR Devaraj - Biologia, 2016 - degruyter.com
MicroRNAs (miRNAs) play key roles in plant responses to biotic and abiotic stresses by
modulating their own expression and a wide array of target mRNAs. Reverse transcription …

Identification of cotton microRNAs and their targets

B Zhang, Q Wang, K Wang, X Pan, F Liu, T Guo… - Gene, 2007 - Elsevier
No study has been performed on identifying microRNAs (miRNAs) and their targets in cotton
although cotton is one of the most important fiber and economic crops around the world. In …

Identification of microRNAs and their targets in four Gossypium species using RNA sequencing

VR Sripathi, Y Choi, ZB Gossett, DM Stelly, EM Moss… - Current Plant …, 2018 - Elsevier
MicroRNAs (miRNAs) are small non-coding endogenous riboregulators that play significant
roles in plant growth, development, metabolism, defense, and stress responses. Upland …

Identification of reference genes for quantitative expression analysis of microRNAs and mRNAs in barley under various stress conditions

J Ferdous, Y Li, N Reid, P Langridge, BJ Shi… - PLoS …, 2015 - journals.plos.org
For accurate and reliable gene expression analysis using quantitative real-time reverse
transcription PCR (qPCR), the selection of appropriate reference genes as an internal …