Optimization of retroviral packaging cells for scale-up of vector production
Y van Heuvel - 2023 - repo.uni-hannover.de
Retroviral vectors (RVVs) are the key players for somatic gene therapy. However, the large-
scale production is very time-and cost-intensive due to the production in adherent cell lines …
scale production is very time-and cost-intensive due to the production in adherent cell lines …
Establishment of a novel stable human suspension packaging cell line producing ecotropic retroviral MLV (PVC-211) vectors efficiently transducing murine …
Y van Heuvel, K Berg, T Hirch, K Winn… - Journal of virological …, 2021 - Elsevier
Retroviral vectors derived from murine leukemia virus (MLV) are amongst the most
frequently utilized vectors in gene therapy approaches such as the genetic modification of …
frequently utilized vectors in gene therapy approaches such as the genetic modification of …
Generation of retroviral packaging and producer cell lines for large-scale vector production and clinical application: improved safety and high titer
PL Sheridan, M Bodner, A Lynn, TK Phuong… - Molecular Therapy, 2000 - cell.com
For many applications, human clinical therapies using retroviral vectors still require many
technological improvements in key areas of vector design and production. These …
technological improvements in key areas of vector design and production. These …
Production of retroviral vectors in continuous high cell density culture
MD Hein, D Kazenmaier, Y van Heuvel, T Dogra… - Applied Microbiology …, 2023 - Springer
Retroviral vectors derived from murine leukemia virus (MLV) are used in somatic gene
therapy applications eg for genetic modification of hematopoietic stem cells. Recently, we …
therapy applications eg for genetic modification of hematopoietic stem cells. Recently, we …
Production scale-up and validation of packaging cell clearance of clinical-grade retroviral vector stocks produced in cell factories
M Przybylowski, A Hakakha, J Stefanski, J Hodges… - Gene Therapy, 2006 - nature.com
The clinical implementation of gene therapy requires large-scale production of viral vector
stocks (VS) derived from packaging cell lines. Upon scaling-up, maintenance of high viral …
stocks (VS) derived from packaging cell lines. Upon scaling-up, maintenance of high viral …
Progress and prospects: advancements in retroviral vector design, generation, and application
S Mukherjee, AJ Thrasher - Human gene therapy, 2011 - liebertpub.com
Although gammaretroviruses have been hugely important for proof-of-principle
demonstrations of efficacy in several specific clinical applications, other retroviral classes …
demonstrations of efficacy in several specific clinical applications, other retroviral classes …
Retroviral vector production for gene therapy applications
S McTaggart - 2001 - etheses.bham.ac.uk
The production of retroviral vectors for gene therapy applications faces a number of
challenges. Of primary concern is the low titre of vector stocks produced by packaging cells …
challenges. Of primary concern is the low titre of vector stocks produced by packaging cells …
Production of Retroviral Vectors
AS Coroadinha, L Gama-Norton… - Current Gene …, 2010 - ingentaconnect.com
Retroviral vectors are presently amongst the most widely used vectors in gene therapy
clinical trials to target pathologies of different origins, such as cancers, genetic diseases or …
clinical trials to target pathologies of different origins, such as cancers, genetic diseases or …
Production of retroviral vectors
AS Coroadinha, L Gama-Norton, AI Amaral… - Current gene …, 2010 - europepmc.org
Retroviral vectors are presently amongst the most widely used vectors in gene therapy
clinical trials to target pathologies of different origins, such as cancers, genetic diseases or …
clinical trials to target pathologies of different origins, such as cancers, genetic diseases or …
[HTML][HTML] Rapid establishment of stable retroviral packaging cells and recombinant susceptible target cell lines employing novel transposon vectors derived from …
K Berg, VN Schäfer, N Bartnicki, R Eggenschwiler… - Virology, 2019 - Elsevier
Viral vector particles derived from murine leukemia virus (MLV) mediate highly efficient
stable gene transfer used in gene therapeutic approaches and in the generation of …
stable gene transfer used in gene therapeutic approaches and in the generation of …