[HTML][HTML] Media optimization to promote rat embryonic development to the blastocyst stage in vitro
Efficient model production in rats that incorporates newly developed genetic editing and
embryo transfer tools, such as CRISPR/Cas9 technology and non-surgical embryo transfer …
embryo transfer tools, such as CRISPR/Cas9 technology and non-surgical embryo transfer …
The effect of supplementation of amino acids and taurine to modified KSOM culture medium on rat embryo development
K Nakamura, K Morimoto, K Shima, Y Yoshimura… - Theriogenology, 2016 - Elsevier
The rat is widely used as a laboratory animal for research. In particular, genetically
engineered rats are essential for production of animal models of several diseases. Although …
engineered rats are essential for production of animal models of several diseases. Although …
The novel use of modified pig zygotic medium for the efficient culture of the preimplantation mouse embryos
D Amarnath, S Wakayama, J Zhu, AR Moawad… - Theriogenology, 2011 - Elsevier
A high potassium concentration in culture media is considered detrimental to in vitro culture
of mouse embryos. Here we show that pig zygotic medium (PZM) containing a higher …
of mouse embryos. Here we show that pig zygotic medium (PZM) containing a higher …
KSOM-R supports both mouse and rat preimplantation embryo development in vitro
A modified KSOM for rat embryo culture (KSOM-R), which has enriched taurine, glycine,
glutamic acid, and alanine, promoted rat embryo development in vitro. Since mice and rats …
glutamic acid, and alanine, promoted rat embryo development in vitro. Since mice and rats …
In vitro development of one-cell embryos from outbred mice: influence of culture medium composition
A Spindle - In vitro cellular & developmental biology, 1990 - Springer
One-cell embryos from outbred mice (CF1, CD-1, and Dub: ICR) were cultured in various
modifications of egg culture medium (ECM). The best development was observed in medium …
modifications of egg culture medium (ECM). The best development was observed in medium …
Systematic analysis of the factors that adversely affect the rate of cell accumulation in mouse embryos during their culture in vitro
XL Jin, C O'Neill - Reproductive Biology and Endocrinology, 2014 - Springer
Background Retarded embryo growth is a pervasive effect of culture in vitro. Methods A
systematic analysis of the interactions between media design, embryo culture density …
systematic analysis of the interactions between media design, embryo culture density …
Development of rabbit zygotes into blastocysts in defined protein-free medium and offspring born following culture and embryo transfer
J Li, RH Foote, Z Liu, JR Giles - Theriogenology, 1997 - Elsevier
We report here on an improved, completely defined culture system for producing embryos in
vitro which mimics development in vivo. This system avoids the confounding effects of the …
vitro which mimics development in vivo. This system avoids the confounding effects of the …
Influence of the storage conditions of embryo culture media on mouse development
M Davenport, Y Wang, LM Fedorov - In Vitro Cellular & Developmental …, 2024 - Springer
The culture of preimplantation embryos in vitro is an important method for human and mouse
reproductive technology. This study aims to investigate the influence of different conditions …
reproductive technology. This study aims to investigate the influence of different conditions …
A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos
AF Ermisch, JR Herrick, R Pasquariello, MKC Dyer… - Scientific reports, 2020 - nature.com
Further refinement of culture media is needed to improve the quality of embryos generated
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …
Microinjection and oviduct transfer procedures for rat model generation with CRISPR-Cas9 technology
TM Alcantar, XY Rairdan - Microinjection: Methods and Protocols, 2019 - Springer
Since the first knockout rat model was generated with zinc-finger nucleases (ZFNs) by
Geurt's group in 2009, the demand for making targeted rat models has increased …
Geurt's group in 2009, the demand for making targeted rat models has increased …