Background CRISPR technology has revolutionized fungal genetic engineering by
increasing the speed and complexity of the experiments that can be performed. Moreover …

Background Within the last years, numerous reports described successful application of the
CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a …

Functional genomics of filamentous fungi has gradually uncovered gene information for
constructing 'cell factories' and controlling pathogens. Available gene manipulation methods …

Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

As a powerful tool for fast and precise genome editing, the CRISPR/Cas9 system has been
applied in filamentous fungi to improve the efficiency of genome alteration. However, the …

CRISPR-Cas9 technologies are revolutionizing fungal gene editing. Here we show that
survival of specific Cas9/sgRNA mediated DNA double strand breaks (DSBs) depends on …

Classic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-
strand breaks for genome editing. To prevent the potential hazard caused by double-strand …

The introduction of CRISPR technologies has revolutionized strain engineering in
filamentous fungi. However, its use in commercial applications has been hampered by …

The CRISPR/Cas9 system is a revolutionary genome editing tool. However, in eukaryotes,
search and optimization of a suitable promoter for guide RNA expression is a significant …