Assisted reproduction is one of the significant tools to treat human infertility. Morphological
assessment is the primary method to determine sperm and embryo viability during in vitro …

In PubMed, it is possible to find more than 40,000 papers on embryo evaluation in various
species. However, there is no consensus or gold standard method on how to assess their …

We propose a swept source-based digital holographic phase microscopy technique. By
scanning source wavelength, a series of on-axis interferograms can be obtained for …

An investigation is reported of the identification and measurement of region of interest (ROI)
in quantitative phase-contrast maps of biological cells by digital holographic microscopy. In …

Embryo quality is a crucial factor affecting live birth outcomes. However, an accurate
diagnostic for embryo quality remains elusive in the in vitro fertilization clinic. Determining …

Assisted reproductive technologies seek to improve the success rate of pregnancies.
Morphology scoring is a common approach to evaluate oocyte and embryo viability prior to …

Most living cells are optically transparent which makes it difficult to visualize them under
bright field microscopy. Use of contrast agents or markers and staining procedures are often …

The morphology of the sperm head has often been correlated with the outcome of in vitro
fertilization, and has been shown to be the sole parameter in semen of value in predicting …

Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells.
Interferometric phase microscopy (IPM) is an optical imaging method based on digital …

Morphology and motility are essential criteria for assessing sperm viability. However, the
human sperm head is small (∼ 3–4 μm) and requires a relatively high-magnification …