Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is …

I Kaasen, P Falkenberg, OB Styrvold… - Journal of …, 1992 - Am Soc Microbiol
It has been shown previously that the otsA and otsB mutations block osmoregulatory
trehalose synthesis in Escherichia coli. We report that the transcription of these …

Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli

HM Giaever, OB Styrvold, I Kaasen… - Journal of …, 1988 - Am Soc Microbiol
It has been shown previously that Escherichia coli accumulates endogenously synthesized
trehalose under osmotic stress. We report here that E. coli contained an osmotically …

Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity

H Li, H Su, SB Kim, YK Chang, SK Hong… - Journal of bioscience …, 2012 - Elsevier
Trehalose production in Escherichia coli DH5α was explored by overexpressing otsBA
operon encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase …

Toluene‐induced accumulation of trehalose by Pseudomonas sp. BCNU 106 through the expression of otsA and otsB homologues

HC Park, YU Bae, SD Cho, SA Kim… - Letters in applied …, 2007 - academic.oup.com
Aim: The objective of this study was to investigate toluene‐induced accumulation
mechanism of trehalose in a toluene‐tolerant bacterium Pseudomonas sp. BCNU 106 …

Trends in bacterial trehalose metabolism and significant nodes of metabolic pathway in the direction of trehalose accumulation

R Ruhal, R Kataria, B Choudhury - Microbial Biotechnology, 2013 - Wiley Online Library
The current knowledge of trehalose biosynthesis under stress conditions is incomplete and
needs further research. Since trehalose finds industrial and pharmaceutical applications …

Synthesis, accumulation, and excretion of trehalose in osmotically stressed Escherichia coli K-12 strains: influence of amber suppressors and function of the …

OB Styrvold, AR Strøm - Journal of bacteriology, 1991 - Am Soc Microbiol
It has been reported previously that Escherichia coli K-12 carries an amber mutation that
prevents osmotic stress-dependent accumulation of trehalose (ML Rod, KY Alam, PR …

Overproduction of trehalose: heterologous expression of Escherichia coli trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in …

L Padilla, R Krämer, G Stephanopoulos… - Applied and …, 2004 - Am Soc Microbiol
Trehalose is a disaccharide with potential applications in the biotechnology and food
industries. We propose a method for industrial production of trehalose, based on improved …

Accumulation of trehalose by Escherichia coli K-12 at high osmotic pressure depends on the presence of amber suppressors

ML Rod, KY Alam, PR Cunningham… - Journal of …, 1988 - Am Soc Microbiol
When grown at high osmotic pressure, some strains of Escherichia coli K-12 synthesized
substantial levels of free sugar and accumulated proline if it was present in the growth …

Osmotic Adaptation of Thermus thermophilus RQ-1: Lesson from a Mutant Deficient in Synthesis of Trehalose

Z Silva, S Alarico, A Nobre, R Horlacher… - Journal of …, 2003 - Am Soc Microbiol
Strains of Thermus thermophilus accumulate primarily trehalose and smaller amounts of
mannosylglycerate in response to salt stress in yeast extract-containing media (OC Nunes …

[PDF][PDF] One step closer to a sweet conclusion

SG Withers, WW Wakarchuk, NCJ Strynadka - Chemistry & biology, 2002 - cell.com
OtsA is required for the biosynthesis of trehalose, a nonreducing disaccharide that is
important for bacterial survival and stress responses. In this issue of Chemistry & Biology …