Advances in sequencing technology allow researchers to map genome-wide changes in
DNA methylation in development and disease. However, there is a lack of experimental …

In mammalian cells, DNA methylation critically regulates gene expression and thus has
pivotal roles in myriad of physiological and pathological processes. Here we report a novel …

Epigenetic studies relied so far on correlations between epigenetic marks and gene
expression pattern. Technologies developed for epigenome editing now enable direct study …

Background DNA methylation has widespread effects on gene expression during
development. However, our ability to assign specific function to regions of DNA methylation …

Despite four decades of research to support the association between DNA methylation and
gene expression, the causality of this relationship remains unresolved. Here, we reaffirm that …

Background Fusion of DNA methyltransferase domains to the nuclease-deficient clustered
regularly interspaced short palindromic repeat (CRISPR) associated protein 9 (dCas9) has …

DNA methylation plays a critical role in the regulation and maintenance of cell-type specific
transcriptional programs. Targeted epigenome editing is an emerging technology to …

In normal mammalian development cytosine methylation is essential and is directed to
specific regions of the genome. Despite notable advances through mapping its genome …

Mammalian genomes exhibit complex patterns of gene expression regulated, in part, by
DNA methylation. The advent of engineered DNA methyltransferases (MTases) to target …

Despite the increased interest in epigenetic research, its progress has been hampered by a
lack of satisfactory tools to control epigenetic factors in specific genomic regions. Until now …