The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate
the activity of genes in eukaryotic cells in diverse settings, varying from basic biological …

The ability to control (trans) gene expression is important both for basic biological research
and applications such as gene therapy. In vivo use of the inducible tetracycline (Tc) …

Background Tight control of gene activity has been achieved in cells and transgenic
organisms using the Tet regulatory systems. Unregulated basal transcription can, however …

The tetracycline (Tc)-dependent system in its" on" version (rtTA system) displays a baseline
activity in the uninduced state, severely limiting its potential applicability in human gene …

The tetracycline (Tet)-dependent regulatory system has been widely used for controlling
gene expression. The Tet-on version of the system, in which the reverse Tet-responsive …

We present viral evolution as a novel and powerful method to optimize non-viral proteins.
We used this approach to optimize the tetracycline (Tc)-regulated gene expression system …

Abstract Doxycycline (Dox) controlled Tet systems provide a powerful and commonly used
method for functional studies on the consequences of gene overexpression/downregulation …

Inducible gene expression based upon Tet repressor (tet regulation) is a broadly applied
tool in molecular genetics. In its original environment, Tet repressor (TetR) negatively …

Be it the B class, or another class of tetracycline (tet) repressor, the utility and specificity of
transcriptional regulators based on this family of prokaryotic DNA binding proteins is …

Regulatory elements that control tetracycline resistance in Escherichia coli were previously
converted into highly specific transcription regulation systems that function in a wide variety …