Packaging functionally important plasma proteins into the α‐granules of human‐induced pluripotent stem cell‐derived megakaryocytes
N Zhang, PJ Newman - Journal of tissue engineering and …, 2019 - Wiley Online Library
The contents of platelet α‐granules arrive via a number of pathways; some are synthesized
by megakaryocytes (MKs), for example, von Willebrand factor (VWF), whereas others are …
by megakaryocytes (MKs), for example, von Willebrand factor (VWF), whereas others are …
Efficient generation of megakaryocytes from human induced pluripotent stem cells using food and drug administration-approved pharmacological reagents
Megakaryocytes (MKs) are rare hematopoietic cells in the adult bone marrow and produce
platelets that are critical to vascular hemostasis and wound healing. Ex vivo generation of …
platelets that are critical to vascular hemostasis and wound healing. Ex vivo generation of …
[HTML][HTML] Gene and protein expression in human megakaryocytes derived from induced pluripotent stem cells
Background There is interest in deriving megakaryocytes (MKs) from pluripotent stem cells
(iPSC) for biological studies. We previously found that genomic structural integrity and …
(iPSC) for biological studies. We previously found that genomic structural integrity and …
[HTML][HTML] Platelet production system using an immortalized megakaryocyte cell line derived from human pluripotent stem cells
S Nakamura, N Takayama, H Nakauchi, K Eto - Blood, 2011 - Elsevier
Abstract MRG-1 Human induced pluripotent stem cells (hiPSCs) are a promising source of
blood cells, including platelets, for transfusion. However, there remains a need for: 1) a …
blood cells, including platelets, for transfusion. However, there remains a need for: 1) a …
[HTML][HTML] Forming megakaryocytes from murine induced pluripotent stem cells by the inducible overexpression of supporting factors
K Cullmann, M Jahn, M Spindler, F Schenk… - Research and Practice …, 2021 - Elsevier
Background Platelets are small anucleate cells that circulate in the blood in a resting state
but can be activated by external cues. In case of need, platelets from blood donors can be …
but can be activated by external cues. In case of need, platelets from blood donors can be …
Identifying and enriching platelet-producing human stem cell–derived megakaryocytes using factor V uptake
Stem cell–derived platelets have the potential to replace donor platelets for transfusion.
Defining the platelet-producing megakaryocytes (MKs) within the heterogeneous MK culture …
Defining the platelet-producing megakaryocytes (MKs) within the heterogeneous MK culture …
Characterization of induced pluripotent stem cell‐derived megakaryocyte lysates for potential regenerative applications
A Baigger, D Eicke, Y Yuzefovych… - Journal of Cellular …, 2018 - Wiley Online Library
Recently, platelet‐derived growth factors present in lysates became an extreme interest in
the field of regenerative medicine such as in wound healing and as substitutes to foetal …
the field of regenerative medicine such as in wound healing and as substitutes to foetal …
[HTML][HTML] Scalable generation of universal platelets from human induced pluripotent stem cells
Q Feng, N Shabrani, JN Thon, H Huo, A Thiel… - Stem cell reports, 2014 - cell.com
Human induced pluripotent stem cells (iPSCs) provide a potentially replenishable source for
the production of transfusable platelets. Here, we describe a method to generate …
the production of transfusable platelets. Here, we describe a method to generate …
[HTML][HTML] Improving human induced pluripotent stem cell-derived megakaryocyte differentiation and platelet production
L Krisch, G Brachtl, S Hochmann, AC Andrade… - International Journal of …, 2021 - mdpi.com
Several protocols exist for generating megakaryocytes (MKs) and platelets from human
induced pluripotent stem cells (hiPSCs) with limited efficiency. We observed previously that …
induced pluripotent stem cells (hiPSCs) with limited efficiency. We observed previously that …
[HTML][HTML] Expandable megakaryocyte cell lines enable clinically applicable generation of platelets from human induced pluripotent stem cells
S Nakamura, N Takayama, S Hirata, H Seo, H Endo… - Cell stem cell, 2014 - cell.com
The donor-dependent supply of platelets is frequently insufficient to meet transfusion needs.
To address this issue, we developed a clinically applicable strategy for the derivation of …
To address this issue, we developed a clinically applicable strategy for the derivation of …