The knowledge of interactions among functional proteins helps researchers understand
disease mechanisms and design potential strategies for treatment. As a general approach …

Exploration of protein function and interaction is critical for discovering links among
genomics, proteomics, and disease state; yet, the immense complexity of proteomics found …

The ability to specifically label proteins with a wide range of optical properties and
functionalities can help reveal information about protein functions and dynamics in living …

Visualizing the structure and dynamics of biomolecules is critical to understand biological
function, and requires methods to fluorescently label targets of interest in their cellular …

www.bio-protocol.org/e2526 Labelling HaloTag Fusion Proteins with HaloTag Ligand in
Living Cells Huy Nguyen Duc and Xiaojun Ren* Page 1 www.bio-protocol.org/e2526 Vol 7 …

Appending proteins of interest to fluorescent protein tags such as GFP has revolutionized
how proteins are studied in the cellular environment. Over the last few decades many …

Visualizing and manipulating the behavior of proteins is crucial to understanding the
physiology of the cell. Methods of biorthogonal protein labeling are important tools to attain …

Among the many molecular imaging techniques, reporter gene imaging has been a dynamic
area of research. The HaloTag protein is a modified haloalkane dehalogenase which was …

Understanding protein function and interaction is central to the elucidation of biological
processes. Systematic analysis of protein interactions have shown that the eukaryotic …

Background: HaloTag is a modified bacterial enzyme that binds rapidly and irreversibly to an
array of synthetic ligands, including chemical dyes. When expressed in live cells in …