Perennial crops, such as fruit trees, are infected by many viruses, which are transmitted
through vegetative propagation and grafting of infected plant material. Some of these …

Conventional detection of viruses and virus-like diseases of plants is accomplished using a
combination of molecular, serological, and biological indexing. These are the primary tools …

High throughput sequencing technologies accelerated the pace of discovery and
identification of new viral species. Nevertheless, biological characterization of a new virus is …

A sensitive and reliable one step RT-PCR reaction with an internal control has been
developed to detect and differentiate eight important viruses that affect stone fruit tress …

Over the last decade, virologists have discovered an unprecedented number of viruses
using high throughput sequencing (HTS), which led to the advancement of our knowledge …

Banana trees, citrus fruit trees, pome fruit trees, grapevines, mango trees, and stone fruit
trees are major fruit trees cultured worldwide and correspond to nearly 90% of the global …

A polyvalent nested RT-PCR test was developed to detect five well characterised
filamentous fruit tree viruses, namely ACLSV, SCGRMV, ASPV, ASGV and CVA. Short …

Background High-throughput sequencing (HTS) has been applied successfully for virus and
viroid discovery in many agricultural crops leading to the current drive to apply this …

Viruses cause a big fraction of economically important diseases in major crops, including
tomato. In the past decade (2011–2020), many emerging or re-emerging tomato-infecting …

Recent studies have shown the superiority of high-throughput sequencing (HTS) technology
over many standard protocols for pathogen detection. HTS was initiated on fruit tree …