Expression of Canine Interferon Alpha in Silkworm-baculovirus Expression System and the Antiviral Activity Assay

H Li, X Hu, Y Yi, X Yang, Z Zhang… - Biotechnology …, 2015 - biotech.aiijournal.com
Canine interferon alpha (CaIFNα) was widely used in the prophylaxis and cure of canine
diseases. The purpose of this study is to develop an efficient method to express CaIFNα. A …

Cloning, expression and antiviral activity of arctic fox (Alopex lagopus) interferon-gamma gene

H Zhang, X Chai, G Luo, F Wang, L Yi… - Sheng wu Gong …, 2008 - europepmc.org
In order to characterize the biological activity of fox (Vulpes vulpes) interferon gamma
(VuIFN-gamma), We have isolated the cDNA encoding arctic fox (Alopex lagopus) VuIFN …

Soluble expression and activity analysis of mallard IFN-Α

L Liu, Y Zhuang, X Yu, X Zeng - Biotechnology Bulletin, 2014 - biotech.aiijournal.com
In order to acquire efficient expression and production of biologically active duck IFN-α, the
mature protein gene of duck IFN-α was amplified from pMD18T-MaIFN-α. The fragment was …

Expression and Antiviral Activity Detection of Porcine Interferon-gamma in Silkworm

X LIU, H LI, X HU, Z ZHANG, Y YI… - Biotechnology …, 2016 - biotech.aiijournal.com
Interferon is used for threating viral infectious diseases and enhancing vaccine efficacy in
stockbreeding. In this study, recombinant porcine interferon-gamma (PoIFNγ) was …

Study on effect of duck gamma interferon against duck enteritis virus on cells in vitro.

LSQ Li SiQi, YHC Yin HaiChang, WYP Wang YiPing… - 2018 - cabidigitallibrary.org
In order to preliminarily explore the antiviral effect of duck IFNγ, duck IFNγ cDNA was
amplified from duck embryo fibroblast (DEF). The duck IFNγ gene was ligated with the …

Prokaryotic expression of Yanbian goose interferon-α gene and biological activities of the expressed product.

PYG Peng YongGang, CHD Chen HaiDi, GX Gao Xu… - 2014 - cabidigitallibrary.org
Based on the vector pMD18-T-IFN-α constructed in our laboratory, an interferon-α (IFN-α)
gene was amplified by PCR, and subcloned into pET-30a. The target protein was expressed …

Study on effect of duck gamma interferon against duck enteritis virus on cells in vitro.

SQ Li, HC Yin, YP Wang, JL Jin, HY Chen… - Chinese Veterinary …, 2018 - cabdirect.org
In order to preliminarily explore the antiviral effect of duck IFNγ, duck IFNγ cDNA was
amplified from duck embryo fibroblast (DEF). The duck IFNγ gene was ligated with the …

Transient expression of goose IFN-α gene in COS-7 cells and primary investigation of its antiviral function.

LF Liu Fei, TWJ Tang WeiJie, CAC Cheng AnChun… - 2011 - cabidigitallibrary.org
To elucidate the character and functions of goose interferon-alpha gene (IFN-α), the gene
IFN-α of Tianfu meat goose open reading frame (ORF) was cloned into eukaryotic …

Expression and activity analysis of recombinant Yangzhou goose ifn-α.

ML Zhu, C Wang, X Cao, XZ Xu, HX Liu, W Hui-Cong… - 2020 - cabidigitallibrary.org
In order to obtain goose interferon with antiviral activity, the primers were designed based on
the goose IFN-α genesequence published in GenBank amplified the whole gene of …

Expression of bovine interferon gamma in recombinant baculovirus and determination of its antiviral activity

LT Qin, XJ Wang, S Hu, SD Liu, ZG Bu - Wei Sheng wu xue bao …, 2007 - europepmc.org
The full-length bovine interferon gamma (BoIFN-gamma) cDNA, including the secretion
signal peptide coding region was recloned into baculovirus honor vectors pFastBac 1 of Bac …