Expression, Purification, and Activity Assay of Chicken Interferon-Alpha

Y Ma, M Long, A Diao - Proceedings of the 2012 International Conference …, 2014 - Springer
In this paper, Chicken alpha interferon (IFN-α) gene was cloned into pHis-NusA expression
vector, then the recombinant expression vector was transformed into host bacteria E. coli …

Soluble expression of chicken interferon-gamma and activity of purified expression product against NDV.

DL Dai Li, SAS Shan AnShan, SJH Sun JinHua… - 2011 - cabidigitallibrary.org
Abstract chIFN-γ mature protein gene was amplified by RT-PCR from recombinant PMD-18T-
preIFN-γ and then cloned into the prokaryotic expression vector pProEXTMHTa …

Antiserum preparation of recombinant duck interferon-gamma.

Y Wang, Y Guo, Z Wu, A Wang, C Guo, S Wu, W Hong… - 2019 - cabidigitallibrary.org
According to the codon preference of Escherichia coli, this study optimized the codon of the
duck interferon-gamma gene sequence published in GenBank, and then ligated it into the …

Preparation and antiviral effect in vitro of recombinant interferon-alpha of Shitou goose.

ZXL Zheng XiaoLing, LYF Liu YanFen… - 2010 - cabidigitallibrary.org
The interferon-α gene of Shitou goose was amplified from genomic DNA and the segment
coding the mature peptide of the GoIFN-α gene was cloned into prokaryotic expression …

Soluble expression of chicken interferon-gamma and antiviral activity of purified expression product

J Qi, Y Du, X Zhu, B Hu, S Sun, X Zhang… - Wei Sheng wu xue bao …, 2009 - europepmc.org
Objective We cloned and expressed chicken interferon-gamma gene (chIFN-gamma), and
detected the bioactivity of chIFN-gamma expressed in Escherichia coli (E. coli). Methods The …

Expression in MDBK cell and detection of the anti-bovine viral diarrhea virus activity of Sika deer interferon beta1.

LQH Liu QianHui, LZ Li Zhe, SFY Su FengYan… - 2016 - cabidigitallibrary.org
In order to express Sika deer interferon beta1 (IFN-β1) and detect the antioxidant activity of
bovine viral diarrhea virus (BVDV), the IFN-β1 gene was amplified by PCR from genome …

Interferon-α Genes from Bos and Bubalus bubalus

X Shi, C Xia, B Pan, M Wang - Animal biotechnology, 2006 - Taylor & Francis
Interferon-α genes were cloned from six breeds of three species of two genera (three
Chinese native cattle breeds of yellow cattle, wild yak and HuanHu domestic yak, one …

Efficient production of canine interferon-alpha in silkworm Bombyx mori by use of a BmNPV/Bac-to-Bac expression system

Z Na, Y Huipeng, L Lipan, C Cuiping… - Applied microbiology …, 2008 - Springer
We exploited the silkworm Bombyx mori for the production of recombinant canine interferon-
alpha (CaIFN-α). The recombinant baculovirus harboring canine interferon gene was rapidly …

Prokaryotic expression, purification and preparation of polyclonal antibody of Chinese Alligator IFN-α.

Y Bai, R Fei - 2022 - cabidigitallibrary.org
In order to further study the Chinese alligator 1FN-α, the prokaryotic expression vector
pETBZa-IFN was constructed and introduced into the Escherichia coli expression system …

Expression and identification of chicken interferon-γ with MultiBac system.

Y Zhou, M Feng, H Yan, J Sun - 2021 - cabidigitallibrary.org
MultiBac system is a complete and powerful eukaryotic expression system that can produce
foreign proteins of high quality and great quantity in insect cells, and has been used in …