Background: Chlamydia trachomatis is a prolific human pathogen that can cause serious
long-term conditions if left untreated. Recent developments in Chlamydia genetics have …

Transposon mutagenesis is a widely applied and powerful genetic tool for the discovery of
genes associated with selected phenotypes. Chlamydia trachomatis is a clinically …

Gene inactivation is essential for forward and reverse genetic approaches to establish
protein function. Techniques such as insertion or chemical mutagenesis have been …

Transposon mutagenesis is a widely applied and powerful genetic tool for the discovery of
genes associated with selected phenotypes. Chlamydia trachomatis is a clinically …

Plasmid transformation of chlamydiae has created new opportunities to investigate host–
microbe interactions during chlamydial infections; however, there are still limitations …

Background C hlamydia spp. are obligate, intracellular bacteria that infect humans and
animals. Research on these important pathogens has been hindered due to a paucity of …

Gene inactivation by transposon insertion or allelic exchange is a powerful approach to
probe gene function. Unfortunately, many microbes, including Chlamydia, are not amenable …

Although progress in Chlamydia genetics has been rapid, genomic modification has
previously been limited to point mutations and group II intron insertions which truncate …

Chlamydia trachomatis is an obligate intracellular pathogen of humans, causing both the
sexually transmitted infection, chlamydia, and the most common cause of infectious …

Our study had three objectives: to extend the plasmid-based transformation protocol to a
clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide “proof of …