Development of a plasmid shuttle vector system for genetic manipulation of Chlamydia psittaci

K Shima, MM Weber, C Schnee, K Sachse, N Käding… - Msphere, 2020 - Am Soc Microbiol
The obligate intracellular bacterium Chlamydia psittaci is a known avian pathogen causing
psittacosis in birds and is capable of zoonotic transmission. In human pulmonary infections …

Transformation of a plasmid-free, genital tract isolate of Chlamydia trachomatis with a plasmid vector carrying a deletion in CDS6 revealed that this …

Y Wang, LT Cutcliffe, RJ Skilton, K Persson… - Pathogens and …, 2013 - academic.oup.com
The development of a plasmid-based genetic transformation protocol for Chlamydia
trachomatis provides the basis for the detailed investigation of the function of the chlamydial …

Generation of targeted Chlamydia trachomatis null mutants

L Kari, MM Goheen, LB Randall… - Proceedings of the …, 2011 - National Acad Sciences
Chlamydia trachomatis is an obligate intracellular bacterial pathogen that infects hundreds
of millions of individuals globally, causing blinding trachoma and sexually transmitted …

Population Genomics of Chlamydia trachomatis: Insights on Drift, Selection, Recombination, and Population Structure

SJ Joseph, X Didelot, J Rothschild… - Molecular biology …, 2012 - academic.oup.com
The large number of sexually transmitted diseases and ocular trachoma cases that are
caused globally each year by Chlamydia trachomatis has made this organism a World …

[HTML][HTML] Transformation of Sexually Transmitted Infection-Causing Serovars of Chlamydia trachomatis Using Blasticidin for Selection

H Ding, S Gong, Y Tian, Z Yang, R Brunham, G Zhong - PloS one, 2013 - journals.plos.org
Plasmid-free Chlamydia trachomatis serovar L2 organisms have been transformed with
chlamydial plasmid-based shuttle vectors pGFP:: SW2 and pBRCT using β-lactamase as a …

Molecular Genetic Analysis of Chlamydia Species

BS Sixt, RH Valdivia - Annual Review of Microbiology, 2016 - annualreviews.org
Species of Chlamydia are the etiologic agent of endemic blinding trachoma, the leading
cause of bacterial sexually transmitted diseases, significant respiratory pathogens, and a …

A minimal replicon enables efficacious, species-specific gene deletion in Chlamydia and extension of gene knockout studies to the animal model of infection using …

KA Fields, MD Bodero, KR Scanlon… - Infection and …, 2022 - Am Soc Microbiol
The genus Chlamydia consists of diverse, obligate intracellular bacteria that infect various
animals, including humans. Although chlamydial species share many aspects of the typical …

Floxed-cassette allelic exchange mutagenesis enables markerless gene deletion in Chlamydia trachomatis and can reverse cassette-induced polar effects

G Keb, R Hayman, KA Fields - Journal of Bacteriology, 2018 - Am Soc Microbiol
As obligate intracellular bacteria, Chlamydia spp. have evolved numerous, likely intricate,
mechanisms to create and maintain a privileged intracellular niche. Recent progress in …

[HTML][HTML] The Impact of Lateral Gene Transfer in Chlamydia

H Marti, RJ Suchland, DD Rockey - Frontiers in cellular and infection …, 2022 - frontiersin.org
Lateral gene transfer (LGT) facilitates many processes in bacterial ecology and
pathogenesis, especially regarding pathogen evolution and the spread of antibiotic …

[HTML][HTML] Chloramphenicol acetyltransferase as a selection marker for chlamydial transformation

S Xu, L Battaglia, X Bao, H Fan - BMC research notes, 2013 - Springer
Background Chlamydia is a common bacterial pathogen responsible for many diseases.
Methods for transforming this important organism using a β-lactamase as a selection marker …