Site-directed spin labeling for EPR studies of nucleic acids

SA Shelke, ST Sigurdsson - Modified Nucleic Acids, 2016 - Springer
Electron paramagnetic resonance (EPR) spectroscopy has emerged as a valuable
technique to study the structure and dynamics of nucleic acids and their complexes with …

Site‐Directed Spin Labelling of Nucleic Acids

SA Shelke, ST Sigurdsson - European Journal of Organic …, 2012 - Wiley Online Library
The study of the structure and dynamics of the nucleic acids and their complexes with other
biomolecules is the basis for understanding their functions. Electron paramagnetic …

Noncovalent and Site‐Directed Spin Labeling of Nucleic Acids

SA Shelke, ST Sigurdsson - Angewandte Chemie International …, 2010 - Wiley Online Library
Electron paramagnetic resonance (EPR) spectroscopy is widely used to study free radicals
or paramagnetic centers associated with biopolymers.[1] With the advent of pulsed EPR …

Nucleic acid structure and dynamics: perspectives from site-directed spin labeling

Y Ding, P Nguyen, NS Tangprasertchai, CV Reyes… - 2014 - books.rsc.org
The technique of site-directed spin labeling (SDSL) provides information on bio-molecular
systems by monitoring the behaviors of a stable radical tag (ie, spin label) using electron …

Site-directed spin labeling of RNA by postsynthetic modification of 2′-amino groups

S Saha, AP Jagtap, ST Sigurdsson - Methods in Enzymology, 2015 - Elsevier
To elucidate mechanisms that govern functions of nucleic acids, it is essential to understand
their structure and dynamics. Electron paramagnetic resonance (EPR) spectroscopy is a …

A versatile approach for site-directed spin labeling and structural EPR studies of RNAs

ES Babaylova, AV Ivanov, AA Malygin… - Organic & …, 2014 - pubs.rsc.org
Site-directed spin labeling (SDSL) is widely applied for structural studies of biopolymers by
electron paramagnetic resonance (EPR). However, SDSL of long RNA sequences still …

Site-directed spin labeling measurements of nanometer distances in nucleic acids using a sequence-independent nitroxide probe

Q Cai, AK Kusnetzow, WL Hubbell… - Nucleic acids …, 2006 - academic.oup.com
In site-directed spin labeling (SDSL), local structural and dynamic information is obtained via
electron paramagnetic resonance (EPR) spectroscopy of a stable nitroxide radical attached …

A facile method for attaching nitroxide spin labels at the 5′ terminus of nucleic acids

GPG Grant, PZ Qin - Nucleic Acids Research, 2007 - academic.oup.com
In site-directed spin labeling (SDSL), a nitroxide moiety containing a stable, unpaired
electron is covalently attached to a specific site within a macromolecule, and structural and …

[PDF][PDF] Enzymatic synthesis of multiple spin-labeled DNA

S Obeid, M Yulikov, G Jeschke, A Marx - Angew. Chem., Int. Ed, 2008 - academia.edu
Electron paramagnetic resonance (EPR) spectroscopy is a widespread technique for the
study of the organizational and dynamic properties of biological macromolecules. Many of …

Structural information from oligonucleotides

R Ward, O Schiemann - Structural Information from Spin-Labels and …, 2013 - Springer
The important role of DNA in biology has been known for a long time, but during the last
decade it has become apparent that also RNA has many more functions than previously …