Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects
RM Lennen, AI Nilsson Wallin, M Pedersen… - Nucleic acids …, 2016 - academic.oup.com
Homologous recombination of single-stranded oligonucleotides is a highly efficient process
for introducing precise mutations into the genome of E. coli and other organisms when …
for introducing precise mutations into the genome of E. coli and other organisms when …
Conditional DNA repair mutants enable highly precise genome engineering
Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial
genome editing. The efficient application of this technique requires the inactivation of the …
genome editing. The efficient application of this technique requires the inactivation of the …
A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species
Currently available tools for multiplex bacterial genome engineering are optimized for a few
laboratory model strains, demand extensive prior modification of the host strain, and lead to …
laboratory model strains, demand extensive prior modification of the host strain, and lead to …
Recombineering and MAGE
TM Wannier, PN Ciaccia, AD Ellington… - Nature Reviews …, 2021 - nature.com
Recombination-mediated genetic engineering, also known as recombineering, is the
genomic incorporation of homologous single-stranded or double-stranded DNA into …
genomic incorporation of homologous single-stranded or double-stranded DNA into …
A mimicking-of-DNA-methylation-patterns pipeline for overcoming the restriction barrier of bacteria
G Zhang, W Wang, A Deng, Z Sun, Y Zhang, Y Liang… - 2012 - journals.plos.org
Genetic transformation of bacteria harboring multiple Restriction-Modification (RM) systems
is often difficult using conventional methods. Here, we describe a mimicking-of-DNA …
is often difficult using conventional methods. Here, we describe a mimicking-of-DNA …
Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
Genome engineering using single-stranded oligonucleotides is an efficient method for
generating small chromosomal and episomal modifications in a variety of host organisms …
generating small chromosomal and episomal modifications in a variety of host organisms …
LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells
TW van Ravesteyn, M Dekker, A Fish… - Proceedings of the …, 2016 - National Acad Sciences
Synthetic single-stranded DNA oligonucleotides (ssODNs) can be used to generate subtle
genetic modifications in eukaryotic and prokaryotic cells without the requirement for prior …
genetic modifications in eukaryotic and prokaryotic cells without the requirement for prior …
Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA
Multiplex automated genome engineering (MAGE) is a powerful technology for in vivo
genome editing that uses synthetic single-stranded DNA (ssDNA) to introduce targeted …
genome editing that uses synthetic single-stranded DNA (ssDNA) to introduce targeted …
Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization
HM Cohen, DS Tawfik, AD Griffiths - … Engineering Design and …, 2004 - academic.oup.com
Engineering the specificity of DNA‐modifying enzymes has proven extremely challenging,
as sequence recognition by these enzymes is poorly understood. Here we used directed …
as sequence recognition by these enzymes is poorly understood. Here we used directed …
Low-mutation-rate, reduced-genome Escherichia coli: an improved host for faithful maintenance of engineered genetic constructs
Background Molecular mechanisms generating genetic variation provide the basis for
evolution and long-term survival of a population in a changing environment. In stable …
evolution and long-term survival of a population in a changing environment. In stable …