Molecular genetic manipulation of micro‐organisms requires the development of plasmid‐
mediated transformation systems that include:(i) infusion of exogenous DNA into recipient …

Advances in genetic transformation techniques have made important contributions to
molecular genetics. Various molecular tools and strategies have been developed for …

Filamentous fungi have been of great interest because of their excellent ability as cell
factories to manufacture useful products for human beings. The development of genetic …

Filamentous fungi have a long-standing tradition as industrial producers of primary and
secondary metabolites. Initially, industrial scientists selected production strains from natural …

The protoplast method for the transformation of filamentous fungi is described in detail, as is
the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged …

Recombinant DNA technology offers one the potential to make well-defined alterations in
the genetic make-up of an organism. The development of this technology was based on two …

In comparison with transformation vectors available for use in E. coli or yeast, there has
been relatively little development of vectors for use in filamentous fungi. For example …

The study of gene function in filamentous fungi is a field of research that has made great
advances in very recent years. A number of transformation and gene manipulation strategies …

Several vectors containing dominant antibiotic resistance markers for the transformation of
filamentous fungi are available. However, most of them lack a recombinant selection system …

A better understanding of fungal biology will facilitate judicious use of beneficial fungi and
will also advance our efforts to control pathogenic fungi. Molecular studies of fungal biology …