[HTML][HTML] Finding the active genes in deep RNA-seq gene expression studies
T Hart, HK Komori, S LaMere, K Podshivalova… - BMC genomics, 2013 - Springer
Background Early application of second-generation sequencing technologies to transcript
quantitation (RNA-seq) has hinted at a vast mammalian transcriptome, including transcripts …
quantitation (RNA-seq) has hinted at a vast mammalian transcriptome, including transcripts …
Uncovering the complexity of transcriptomes with RNA‐Seq
In recent years, the introduction of massively parallel sequencing platforms for Next
Generation Sequencing (NGS) protocols, able to simultaneously sequence hundred …
Generation Sequencing (NGS) protocols, able to simultaneously sequence hundred …
Defining transcribed regions using RNA-seq
Next-generation sequencing technologies are revolutionizing genomics research. It is now
possible to generate gigabase pairs of DNA sequence within a week without time …
possible to generate gigabase pairs of DNA sequence within a week without time …
Synthetic spike-in standards for RNA-seq experiments
L Jiang, F Schlesinger, CA Davis, Y Zhang… - Genome …, 2011 - genome.cshlp.org
High-throughput sequencing of cDNA (RNA-seq) is a widely deployed transcriptome
profiling and annotation technique, but questions about the performance of different …
profiling and annotation technique, but questions about the performance of different …
Transcriptome sequencing: RNA-seq
H Zhang, L He, L Cai - Computational Systems Biology: Methods and …, 2018 - Springer
RNA sequencing (RNA-seq) can not only be used to identify the expression of common or
rare transcripts but also in the identification of other abnormal events, such as alternative …
rare transcripts but also in the identification of other abnormal events, such as alternative …
Full-length RNA-seq from single cells using Smart-seq2
Emerging methods for the accurate quantification of gene expression in individual cells hold
promise for revealing the extent, function and origins of cell-to-cell variability. Different high …
promise for revealing the extent, function and origins of cell-to-cell variability. Different high …
Design and validation issues in RNA-seq experiments
Z Fang, X Cui - Briefings in bioinformatics, 2011 - academic.oup.com
The next-generation sequencing technologies are being rapidly applied in biological
research. Tens of millions of short sequences generated in a single experiment provide us …
research. Tens of millions of short sequences generated in a single experiment provide us …
RNA-seq: an assessment of technical reproducibility and comparison with gene expression arrays
Ultra-high-throughput sequencing is emerging as an attractive alternative to microarrays for
genotyping, analysis of methylation patterns, and identification of transcription factor binding …
genotyping, analysis of methylation patterns, and identification of transcription factor binding …
[HTML][HTML] IVT-seq reveals extreme bias in RNA sequencing
NF Lahens, IH Kavakli, R Zhang, K Hayer, MB Black… - Genome biology, 2014 - Springer
Background RNA-seq is a powerful technique for identifying and quantifying transcription
and splicing events, both known and novel. However, given its recent development and the …
and splicing events, both known and novel. However, given its recent development and the …
RNA-Seq and its applications: a new technology for transcriptomics
YX Qi, YB Liu, WH Rong - Yi Chuan= Hereditas, 2011 - europepmc.org
The transcriptome is the complete set of transcripts for certain type of cells or tissues in a
specific developmental stage or physiological condition. Transcriptome analysis can provide …
specific developmental stage or physiological condition. Transcriptome analysis can provide …