Our study had three objectives: to extend the plasmid-based transformation protocol to a
clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide “proof of …

The development of a plasmid-based genetic transformation protocol for Chlamydia
trachomatis provides the basis for the detailed investigation of the function of the chlamydial …

The recent success in transformation of Chlamydia trachomatis represents a major
advancement in Chlamydia research. Plasmid-free C. trachomatis serovar L2 organisms can …

Chlamydia trachomatis remains one of the few major human pathogens for which there is no
transformation system. C. trachomatis has a unique obligate intracellular developmental …

The development of genetic transformation technology for Chlamydia trachomatis using its
endogenous plasmid has recently been described. Chlamydia muridarum cannot be …

The native plasmid of both Chlamydia muridarum and Chlamydia trachomatis has been
shown to control virulence and infectivity in mice and in lower primates. We recently …

Transformation of Chlamydia trachomatis should greatly advance the chlamydial research.
However, significant progress has been hindered by the failure of C. trachomatis to induce …

Chlamydia trachomatis and C. muridarum are human and mouse pathogens, respectively,
which show high conservation of gene order and content. Both species contain a common …

Abstract The 7.5-kb plasmid of Chlamydia trachomatis (CT) is believed to encode essential
genes and might have a role in CT pathogenicity. Accordingly, analysis of plasmid-linked …

Plasmid transformation of chlamydiae has created new opportunities to investigate host–
microbe interactions during chlamydial infections; however, there are still limitations …