Molecular tools for gene manipulation in filamentous fungi

S Wang, H Chen, X Tang, H Zhang, W Chen… - Applied microbiology …, 2017 - Springer
Functional genomics of filamentous fungi has gradually uncovered gene information for
constructing 'cell factories' and controlling pathogens. Available gene manipulation methods …

CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective

R Song, Q Zhai, L Sun, E Huang, Y Zhang… - Applied microbiology …, 2019 - Springer
Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

[HTML][HTML] CRISPR-Cas9 genome editing approaches in filamentous fungi and oomycetes

M Schuster, R Kahmann - Fungal Genetics and Biology, 2019 - Elsevier
Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

MJ Kwon, T Schütze, S Spohner, S Haefner… - Fungal biology and …, 2019 - Springer
Background Within the last years, numerous reports described successful application of the
CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a …

Cpf1 enables fast and efficient genome editing in Aspergilli

KG Vanegas, ZD Jarczynska, T Strucko… - Fungal Biology and …, 2019 - Springer
Background CRISPR technology has revolutionized fungal genetic engineering by
increasing the speed and complexity of the experiments that can be performed. Moreover …

CRISPR/Cas9-based genome editing of the filamentous fungi: the state of the art

TQ Shi, GN Liu, RY Ji, K Shi, P Song, LJ Ren… - Applied microbiology …, 2017 - Springer
In recent years, a variety of genetic tools have been developed and applied to various
filamentous fungi, which are widely applied in agriculture and the food industry. However …

CRISPR/Cas9‐based genome engineering: A new breakthrough in the genetic manipulation of filamentous fungi

M Ullah, L Xia, S Xie, S Sun - Biotechnology and Applied …, 2020 - Wiley Online Library
Filamentous fungi have several industrial, environmental, and medical applications.
However, they are rarely utilized owing to the limited availability of full‐genome sequences …

Progress and challenges: development and implementation of CRISPR/Cas9 technology in filamentous fungi

Q Wang, JJ Coleman - Computational and structural biotechnology journal, 2019 - Elsevier
Widely distributed in various environmental niches, filamentous fungi play an important role
in industry, drug development, and plant/animal health. Manipulation of the genome and the …

A CRISPR-Cas9 system for genetic engineering of filamentous fungi

CS Nødvig, JB Nielsen, ME Kogle, UH Mortensen - PloS one, 2015 - journals.plos.org
The number of fully sequenced fungal genomes is rapidly increasing. Since genetic tools
are poorly developed for most filamentous fungi, it is currently difficult to employ genetic …

Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

TM van Leeuwe, M Arentshorst, T Ernst, E Alazi… - Fungal biology and …, 2019 - Springer
Background CRISPR/Cas9 mediated genome editing has expedited the way of constructing
multiple gene alterations in filamentous fungi, whereas traditional methods are time …