Hematopoietic stem cells (HSCs) represent an important target cell population in bone
marrow transplantation, cell and gene therapy applications, and the development of …

Retroviral-mediated gene transfer is widely used to express proteins in hematopoietic cells
for the analysis of their effects on blood cell proliferation, differentiation, and biological …

Gene therapy of haematopoietic stem cells (HSCs) has been under investigation for 15
years. HSCs can be easily transduced with retroviral vectors (Moloney murine leukaemia …

The ability to efficiently transduce hematopoietic stem cells (HSC) represents a powerful
methodology by which to study the role of specific genes on HSC function, as well as to …

Since the mid-1980s, murine retroviral vectors have been used extensively by a number of
investigators to clonally mark and genetically modify primitive hematopoietic stem cells …

Recombinant retroviruses offer many advantages for the genetic modification of human
hematopoietic cells, although their use in clinical protocols has thus far given disappointing …

Hematopoietic stem cells (HSCs) are desirable targets for gene therapy because of their self-
renewal and multilineage differentiation abilities. Retroviral vectors are extensively used for …

Inefficient retroviral-mediated gene transfer to human hematopoietic stem cells (HSC) and
insufficient gene expression in progeny cells derived from transduced HSC are two major …

A variety of genetic and acquired diseases could conceivably be treated by gene therapy
targeted to hematopoietic stem cells (HSC). Inevitably, the effort to develop reliable methods …

Among the currently available methods for gene transfer, recombinant murine retroviruses
remain the best established method for achieving stable integration of a transgene with high …