Appending proteins of interest to fluorescent protein tags such as GFP has revolutionized
how proteins are studied in the cellular environment. Over the last few decades many …

Exploration of protein function and interaction is critical for discovering links among
genomics, proteomics, and disease state; yet, the immense complexity of proteomics found …

www.bio-protocol.org/e2526 Labelling HaloTag Fusion Proteins with HaloTag Ligand in
Living Cells Huy Nguyen Duc and Xiaojun Ren* Page 1 www.bio-protocol.org/e2526 Vol 7 …

Mass cytometry permits the high dimensional analysis of complex biological samples;
however, some techniques are not yet integrated into the mass cytometry workflow due to …

The ability to specifically label proteins with a wide range of optical properties and
functionalities can help reveal information about protein functions and dynamics in living …

The self-labeling protein HaloTag has been used extensively to determine the localization
and turnover of proteins of interest at the single-cell level. To this end, halogen-substituted …

Understanding protein function and interaction is central to the elucidation of biological
processes. Systematic analysis of protein interactions have shown that the eukaryotic …

Visualizing the structure and dynamics of biomolecules is critical to understand biological
function, and requires methods to fluorescently label targets of interest in their cellular …

Many fluorescent sensors are currently available for in vitro bio-physiological microscopic
imaging. The ability to label cells in living animals with these fluorescent sensors would help …

Recombinant proteins are often expressed with an additional polypeptide the role of which
is to aid purification, detection or functional studies. Recently, the role of these tags has been …