[HTML][HTML] Suppl 1: HaloTag® Platform: From Proteomics to Cellular Analysis and Animal Imaging

M Cong - Current Chemical Genomics, 2012 - ncbi.nlm.nih.gov
From in vitro proteomic protein analysis to in vivo animal models, various protein tagging
systems are designed to provide useful functionalities. Commonly used affinity tags for …

[引用][C] How to Construct and Use a HaloTag о Coding Region Control Expression Vector

S Ananthanarayanan, R Hurst, J Hartnett, M Slater

[HTML][HTML] Suppl 1: In Vivo Fluorescent Labeling of Tumor Cells with the HaloTag® Technology

JC Tseng, HA Benink, MG McDougall… - Current chemical …, 2012 - ncbi.nlm.nih.gov
Many fluorescent sensors are currently available for in vitro bio-physiological microscopic
imaging. The ability to label cells in living animals with these fluorescent sensors would help …

[引用][C] In Vivo Fluorescent Labeling of Tumor Cells with the HaloTag® Technology

HA Benink, MG McDougall, AL Kung, JC Tseng… - 2012 - Bentham Open

[引用][C] Combining Promega HaloTag® Technology with PerkinElmer High Content Imaging Systems

H Benink, K Boettcher

Evaluation of the cytosolic uptake of HaloTag using a pH-sensitive dye

JLB Giancola, JB Grimm, JV Jun, YD Petri… - ACS Chemical …, 2024 - ACS Publications
The efficient cytosolic delivery of proteins is critical for advancing novel therapeutic
strategies. Current delivery methods are severely limited by endosomal entrapment, and …

HaloTag‐Based Reporters for Fluorescence Imaging and Biosensing

A Cook, F Walterspiel, C Deo - ChemBioChem, 2023 - Wiley Online Library
Visualizing the structure and dynamics of biomolecules is critical to understand biological
function, and requires methods to fluorescently label targets of interest in their cellular …

In vivo pretargeted imaging of HER2 and TAG-72 expression using the HaloTag enzyme

JC Knight, M Mosley, HT Uyeda, M Cong… - Molecular …, 2017 - ACS Publications
A novel pretargeted SPECT imaging strategy based on the HaloTag enzyme has been
evaluated for the first time in a living system. To determine the efficacy of this approach, two …

Purification of recombinant proteins from cultured mammalian cells by HaloTag technology

RF Ohana, R Hurst - Current Protocols in Molecular Biology, 2015 - Wiley Online Library
Cultured mammalian cells provide an environment ideal for producing functional
recombinant mammalian proteins. However, low expression levels of recombinant proteins …

In vivo pretargeted dual-modality imaging of HER2 and TAG-72 expression using the HaloTag enzyme

J Knight, M Mosley, M Stratford, HT Uyeda, M Cong… - 2016 - Soc Nuclear Med
1210 Objectives The covalent bond-forming reaction that occurs between the dehalogenase
enzyme HaloTag and a chloroalkane HaloTag ligand (HTL) has been shown to proceed …