Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx

GA Suárez, BA Renda, A Dasgupta… - Applied and …, 2017 - Am Soc Microbiol
The genomes of most bacteria contain mobile DNA elements that can contribute to
undesirable genetic instability in engineered cells. In particular, transposable insertion …

Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution

BA Renda, A Dasgupta, D Leon… - Journal of bacteriology, 2015 - Am Soc Microbiol
Acinetobacter baylyi ADP1 has the potential to be a versatile bacterial host for synthetic
biology because it is naturally transformable. To examine the genetic reliability of this …

Acinetobacter baylyi ADP1: Transforming the choice of model organism

KT Elliott, EL Neidle - IUBMB life, 2011 - Wiley Online Library
For more than 25 years, Acinetobacter baylyi ADP1 has been used in molecular biology
studies that address a broad range of questions. Recently, the rapid accumulation of data …

Acinetobacter baylyi ADP1—naturally competent for synthetic biology

S Santala, V Santala - Essays in Biochemistry, 2021 - portlandpress.com
Acinetobacter baylyi ADP1 is a non-pathogenic soil bacterium known for its metabolic
diversity and high natural transformation and recombination efficiency. For these features, A …

Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering

D Metzgar, JM Bacher, V Pezo, J Reader… - Nucleic acids …, 2004 - academic.oup.com
Acinetobacter sp. strain ADP1 is a naturally transformable gram-negative bacterium with
simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb …

Analysis of IS1236-Mediated Gene Amplification Events in Acinetobacter baylyi ADP1

LE Cuff, KT Elliott, SC Seaton, MK Ishaq… - Journal of …, 2012 - Am Soc Microbiol
Recombination between insertion sequence copies can cause genetic deletion, inversion,
or duplication. However, it is difficult to assess the fraction of all genomic rearrangements …

Natural transformation as a tool in Acinetobacter baylyi: Streamlined engineering and mutational analysis

SR Bedore, EL Neidle, I Pardo, J Luo, AC Baugh… - Methods in …, 2023 - Elsevier
Natural transformation and homologous recombination in a soil bacterium, Acinetobacter
baylyi ADP1, occur with exceptionally high efficiency. These genetic features can be …

Natural transformation as a tool in Acinetobacter baylyi: Evolution by amplification of gene copy number

I Pardo, SR Bedore, MP Tumen-Velasquez… - Methods in …, 2023 - Elsevier
For many years, the natural competency of Acinetobacter baylyi ADP1 facilitated studies of
bacterial metabolism, biochemistry, and physiology. With the advent of synthetic biology …

Expression vectors for Acinetobacter baylyi ADP1

CD Murin, K Segal, A Bryksin… - Applied and …, 2012 - Am Soc Microbiol
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous
recombination, so it can be transformed without restriction digests or ligation reactions …

[HTML][HTML] Costs and benefits of natural transformation in Acinetobacter baylyi

N Hülter, V Sørum, K Borch-Pedersen, MM Liljegren… - BMC microbiology, 2017 - Springer
Background Natural transformation enables acquisition of adaptive traits and drives genome
evolution in prokaryotes. Yet, the selective forces responsible for the evolution and …