Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx
The genomes of most bacteria contain mobile DNA elements that can contribute to
undesirable genetic instability in engineered cells. In particular, transposable insertion …
undesirable genetic instability in engineered cells. In particular, transposable insertion …
Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution
Acinetobacter baylyi ADP1 has the potential to be a versatile bacterial host for synthetic
biology because it is naturally transformable. To examine the genetic reliability of this …
biology because it is naturally transformable. To examine the genetic reliability of this …
Acinetobacter baylyi ADP1: Transforming the choice of model organism
KT Elliott, EL Neidle - IUBMB life, 2011 - Wiley Online Library
For more than 25 years, Acinetobacter baylyi ADP1 has been used in molecular biology
studies that address a broad range of questions. Recently, the rapid accumulation of data …
studies that address a broad range of questions. Recently, the rapid accumulation of data …
Acinetobacter baylyi ADP1—naturally competent for synthetic biology
Acinetobacter baylyi ADP1 is a non-pathogenic soil bacterium known for its metabolic
diversity and high natural transformation and recombination efficiency. For these features, A …
diversity and high natural transformation and recombination efficiency. For these features, A …
Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering
D Metzgar, JM Bacher, V Pezo, J Reader… - Nucleic acids …, 2004 - academic.oup.com
Acinetobacter sp. strain ADP1 is a naturally transformable gram-negative bacterium with
simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb …
simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb …
Analysis of IS1236-Mediated Gene Amplification Events in Acinetobacter baylyi ADP1
LE Cuff, KT Elliott, SC Seaton, MK Ishaq… - Journal of …, 2012 - Am Soc Microbiol
Recombination between insertion sequence copies can cause genetic deletion, inversion,
or duplication. However, it is difficult to assess the fraction of all genomic rearrangements …
or duplication. However, it is difficult to assess the fraction of all genomic rearrangements …
Natural transformation as a tool in Acinetobacter baylyi: Streamlined engineering and mutational analysis
Natural transformation and homologous recombination in a soil bacterium, Acinetobacter
baylyi ADP1, occur with exceptionally high efficiency. These genetic features can be …
baylyi ADP1, occur with exceptionally high efficiency. These genetic features can be …
Natural transformation as a tool in Acinetobacter baylyi: Evolution by amplification of gene copy number
I Pardo, SR Bedore, MP Tumen-Velasquez… - Methods in …, 2023 - Elsevier
For many years, the natural competency of Acinetobacter baylyi ADP1 facilitated studies of
bacterial metabolism, biochemistry, and physiology. With the advent of synthetic biology …
bacterial metabolism, biochemistry, and physiology. With the advent of synthetic biology …
Expression vectors for Acinetobacter baylyi ADP1
CD Murin, K Segal, A Bryksin… - Applied and …, 2012 - Am Soc Microbiol
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous
recombination, so it can be transformed without restriction digests or ligation reactions …
recombination, so it can be transformed without restriction digests or ligation reactions …
[HTML][HTML] Costs and benefits of natural transformation in Acinetobacter baylyi
Background Natural transformation enables acquisition of adaptive traits and drives genome
evolution in prokaryotes. Yet, the selective forces responsible for the evolution and …
evolution in prokaryotes. Yet, the selective forces responsible for the evolution and …