Cryopreserved storage of clonal germplasm in the USDA National Plant Germplasm System

MM Jenderek, BM Reed - In Vitro Cellular & Developmental Biology-Plant, 2017 - Springer
Abstract The US Department of Agriculture-Agricultural Research Service (USDA-ARS),
National Plant Germplasm System (NPGS) plant collections are a critical source of genetic …

[PDF][PDF] Implementing cryogenic storage of clonally propagated plants

BM Reed - Cryo-Letters, 2001 - researchgate.net
Methodology for plant germplasm cryopreservation was created in the 1970s, expanded in
the 1980s, and implemented at the end of the 20th century. Translating experimental …

Implementing cryopreservation for long-term germplasm preservation in vegetatively propagated species

BM Reed - Cryopreservation of plant germplasm II, 2002 - Springer
Vegetatively propagated fruit, vegetables, or forestry related crops are important in the
agriculture of every country. Maintaining the genetic diversity of these crops is more …

[PDF][PDF] Application of cryopreservation protocols at a clonal genebank

BM Reed, J DeNoma, Y Chang - JIRCAS/IPGRI joint international …, 2000 - researchgate.net
For the past 20 years scientists have talked about the promise of using cryopreservation as a
long-term storage technique (Withers 1980, 1991; Engelmann 1991). Now cryopreservation …

Noncryogenic, long-term germplasm storage

A Golmirzaie, J Toledo - Plant Cell Culture Protocols, 1999 - Springer
Germplasm conservation is an important goal in the preservation of genetic diversity.
Conventional methods, such as field and seed conservation, are used for most crops …

Cryopreservation of plant germplasm: introduction and some observations

LE Towill - Cryopreservation of plant germplasm II, 2002 - Springer
It has been amply stated that preservation of germplasm is important not only for plant
improvement and utilization for food, fiber, medicinal and forest crops, but also for …

Cryopreservation of plant cell, tissue, and organ culture for the conservation of germplasm and biodiversity

YPS Bajaj - Cryopreservation of plant germplasm I, 1995 - Springer
The indiscriminate clearing of forests and agricultural land has led to the depeletion of the
naturally occurring reservoirs of plant germplasm. About 20000 species of higher plants are …

In vitro conservation of plant germplasm

PE Rajasekharan, L Sahijram - Plant biology and biotechnology: volume II …, 2015 - Springer
Biotechnology has brought about a revolution in the way that plant genetic resources can be
utilized. Clonal crops cover a wide range of species from the root and tuber crops, such as …

Medium and long-term storage of in vitro cultures of temperate fruit and nut crops

BM Reed, Y Chang - Conservation of plant genetic resources in vitro, 1997 - ars.usda.gov
Research in slow-growth and cryopreservation, storage at liquid nitrogen temperatures, has
greatly advanced in the last decade. These storage procedures are now defined for many …

Biobanking of vegetable genetic resources by in vitro conservation and cryopreservation

C Ruta, M Lambardi, EA Ozudogru - Biodiversity and Conservation, 2020 - Springer
Today, application of in vitro culture by means of slow growth storage of shoot cultures and
cryopreservation of organs, tissues and cells in liquid nitrogen presents a remarkable …