Stain‐free interferometric phase microscopy correlation with DNA fragmentation stain in human spermatozoa

I Barnea, L Karako, SK Mirsky, M Levi… - Journal of …, 2018 - Wiley Online Library
Acridine orange (AO) staining is used to diagnose DNA fragmentation status in sperm cells.
Interferometric phase microscopy (IPM) is an optical imaging method based on digital …

[HTML][HTML] Interferometric phase microscopy for label-free morphological evaluation of sperm cells

M Haifler, P Girshovitz, G Band, G Dardikman… - Fertility and sterility, 2015 - Elsevier
Objective To compare label-free interferometric phase microscopy (IPM) to label-free and
label-based bright-field microscopy (BFM) in evaluating sperm cell morphology. This …

Automated analysis of individual sperm cells using stain‐free interferometric phase microscopy and machine learning

SK Mirsky, I Barnea, M Levi, H Greenspan… - Cytometry Part …, 2017 - Wiley Online Library
Currently, the delicate process of selecting sperm cells to be used for in vitro fertilization
(IVF) is still based on the subjective, qualitative analysis of experienced clinicians using non …

Individual sperm selection by microfluidics integrated with interferometric phase microscopy

PJ Eravuchira, SK Mirsky, I Barnea, M Levi, M Balberg… - Methods, 2018 - Elsevier
The selection of sperm cells possessing normal morphology and motility is crucial for many
assisted reproductive technologies (ART), especially for intracytoplasmic sperm injection …

Topography and refractometry of sperm cells using spatial light interference microscopy

L Liu, ME Kandel, M Rubessa… - … of biomedical optics, 2018 - spiedigitallibrary.org
Characterization of spermatozoon viability is a common test in treating infertility. Recently, it
has been shown that label-free, phase-sensitive imaging can provide a valuable alternative …

Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos

MB Wheeler, RAC Rabel, M Rubessa… - Biology of …, 2024 - academic.oup.com
Assisted reproduction is one of the significant tools to treat human infertility. Morphological
assessment is the primary method to determine sperm and embryo viability during in vitro …

Advanced methods in reproductive medicine: Application of optical nanoscopy, artificial intelligence-assisted quantitative phase microscopy and mitochondrial DNA …

D Popova - 2021 - munin.uit.no
Declined fertility rate and population is a matter of serious concern, especially in the
developed nations. Assisted Reproductive Technologies (ART), including in vitro fertilization …

Identification of bovine sperm head for morphometry analysis in quantitative phase-contrast holographic microscopy

P Memmolo, G Di Caprio, C Distante, M Paturzo… - Optics express, 2011 - opg.optica.org
An investigation is reported of the identification and measurement of region of interest (ROI)
in quantitative phase-contrast maps of biological cells by digital holographic microscopy. In …

[HTML][HTML] Partially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress …

V Dubey, D Popova, A Ahmad, G Acharya, P Basnet… - Scientific reports, 2019 - nature.com
Semen quality assessed by sperm count and sperm cell characteristics such as morphology
and motility, is considered to be the main determinant of men's reproductive health …

Digital holographic microscopy in human sperm imaging

I Crha, J Zakova, M Huser, P Ventruba… - Journal of assisted …, 2011 - Springer
Purpose The aim of this study was to use digital holographic microscopy (DHM) in human
sperm imaging and compare quantitative phase contrast of sperm heads in …