Abstract The National Gene Vector Laboratory (NGVL) is a US National Institutes of Health
initiative charged with providing clinical grade vectors for gene therapy trials. The program …

Background Murine Leukemia Virus (MLV) is a rodent gammaretrovirus that serves as the
backbone for common gene delivery tools designed for experimental and therapeutic …

The clinical implementation of gene therapy requires large-scale production of viral vector
stocks (VS) derived from packaging cell lines. Upon scaling-up, maintenance of high viral …

The success of experimental gene therapy is dependent on the ability to safely and
efficiently introduce transgenes into the target cell or tissue. Retroviral-based vectors …

Murine leukemia virus (MuLV) can be pseudotyped with a variant of the human
immunodeficency virus (HIV) envelope gene encoding the surface glycoprotein gp120-SU …

Abstract Interleukin-2 (IL-2) is a cytokine that induces the proliferation of certain IL-2 receptor
expressing quiescent cells. Human IL-2 was fused to the amino-terminus of amphotropic …

Some aspects of retroviral biology make recombinant retroviruses particularly suitable for
delivering foreign DNA. Most such vectors in use for gene delivery to mammalian cells are …

The development of retroviral vectors has provided scientists with an effective way of
achieving gene transfer stable over cell generations. This is possible because integration of …

Abstract Background An Erratum has been published for this article in Journal of Gene
Medicine 7 (6), 2005, 835. A stable packaging cell line facilitates large‐scale lentivirus …

Lentiviral vectors have undergone several generations of design improvement to enhance
their biosafety and expression characteristics, and have been approved for use in human …