[HTML][HTML] Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of Aspergillus species in the Human Airway
TY Poh, NABM Ali, LLY Chan, PY Tiew… - International journal of …, 2020 - mdpi.com
Background: Prior studies illustrate the presence and clinical importance of detecting
Aspergillus species in the airways of patients with chronic respiratory disease. Despite this …
Aspergillus species in the airways of patients with chronic respiratory disease. Despite this …
Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR
A Alanio, A Sturny-Leclère, M Benabou… - Journal of …, 2016 - Elsevier
Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to
86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than …
86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than …
[HTML][HTML] Molecular detection of Aspergillus in sputum of patients with lower respiratory tract infections
Background: Raised incidences of respiratory tract infections due to fungal agents in
immunocompetent individuals are a cause of concern due to the unavailability of rapid …
immunocompetent individuals are a cause of concern due to the unavailability of rapid …
[HTML][HTML] Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis
Y Shen, X Zhang, W Lin, C Wan, Q Li, Y Jiang - Scientific Reports, 2017 - nature.com
Conventional cytopathology examination of respiratory samples can aid in identifying
Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid …
Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid …
[HTML][HTML] Aspergillus fumigatus Detection and Risk Factors in Patients with COPD–Bronchiectasis Overlap
S Everaerts, K Lagrou, K Vermeersch… - International journal of …, 2018 - mdpi.com
The role of Aspergillus fumigatus in the airways of chronic obstructive pulmonary disease
(COPD) patients with bronchiectasis is currently unclear. We searched for a sensitive and …
(COPD) patients with bronchiectasis is currently unclear. We searched for a sensitive and …
[HTML][HTML] Molecular characterization of Aspergillus infections in an Iranian educational hospital using RAPD-PCR method
K Diba, K Makhdoomi, H Mirhendi - Iranian journal of basic medical …, 2014 - ncbi.nlm.nih.gov
Objective (s): The nosocomial infections by Aspergillus species are associated with
constructions and increased dust loads in hospital indoors. Our main object was to find the …
constructions and increased dust loads in hospital indoors. Our main object was to find the …
[HTML][HTML] A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection
GL Johnson, DF Bibby, S Wong, SG Agrawal… - PloS one, 2012 - journals.plos.org
The polymerase chain reaction (PCR) is widely used as a diagnostic tool in clinical
laboratories and is particularly effective for detecting and identifying infectious agents for …
laboratories and is particularly effective for detecting and identifying infectious agents for …
[HTML][HTML] Evaluation of two commercial real-time PCR kits for Aspergillus DNA detection in bronchoalveolar lavage fluid in patients with invasive pulmonary …
J Denis, F Forouzanfar, R Herbrecht… - The Journal of Molecular …, 2018 - Elsevier
Invasive pulmonary aspergillosis (IPA) is a common complication of immunosuppression.
Rapid diagnosis using molecular techniques is essential to improve patient survival. PCR …
Rapid diagnosis using molecular techniques is essential to improve patient survival. PCR …
Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification
Rapid and quantitative detection of Aspergillus from clinical samples may facilitate an early
diagnosis of invasive pulmonary aspergillosis (IPA). As nucleic acid-based detection is a …
diagnosis of invasive pulmonary aspergillosis (IPA). As nucleic acid-based detection is a …
[HTML][HTML] Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid
PD Khot, DL Ko, RC Hackman, DN Fredricks - BMC infectious diseases, 2008 - Springer
Background The diagnosis of invasive pulmonary aspergillosis (IPA) remains challenging.
Culture and histopathological examination of bronchoalveolar lavage (BAL) fluid are useful …
Culture and histopathological examination of bronchoalveolar lavage (BAL) fluid are useful …
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