Publisher Summary The occurrence of polyadenylate in many eukaryotic mRNAs has
proved a useful handle for isolating various messages from total cellular RNA by molecular …

Degree: MS DegreeYear: 1981 Institute: The University of Texas at Dallas This dissertation
presents a five step generalized scheme of the processing events involved in the production …

Polyadenylated RNA was isolated from nuclei of cultured Drosophila cells, Schneider's line
2, and used as a template to synthesize a complementary DNA probe. Hybridization …

When I was asked for personal reflections on the last twenty years of RNA research, it
occurred to me that, although many of us will say that we work on RNA, what we really work …

Pulse-labeled RNA isolated from E. coli cells grown on limiting phosphate medium and
phosphate-containing medium was analyzed by oligo (dT)-cellulose chromatography and by …

Large quantities of nonpolyadenylated [poly (A (-))] 4 to 18 S RNA were isolated from the
polyribosomes of S phase HeLa S3 cells and were fractionated into multiple discrete RNA …

Most eukaryotic mRNAs have a sequence of polyadenylic acid [poly (A)] at their 3′-termini.
These poly (A) tails are normally added post-transcriptionally in the nucleus with an initial …

Accumulation of [3H] adenine in the acid-soluble pool and in nuclear and cytoplasmic poly
(A) of HeLa cells shows that the nuclear poly (A) rises along a curve similar to that of the acid …

THE presence of polyadenylic acid (poly (A)) tracts in both the heterogeneous nuclear RNA
(HnRNA) and polysomal messenger RNA of eukaryotic cells indicates that the high …

Since 1972, we have used the rabbit globin mRNAs as a model system to develop general
methods for the sequence analysis of polyadenylylated mRNAs (1–3). As starting material …