Cloning and Expression of Chinese Duck Interferon-gamma Gene.

JE Long, LN Huang, WY Wang, MJ Cheng… - … wu wu li xue bao Acta …, 2001 - europepmc.org
The efficacy of cytokine therapy has been demonstrated in several viral diseases. Interferon-
gamma is a cytokine that has potent antiviral property and immunomodulatory activity. To …

Expression and antiviral activity of recombinant duck γ-interferon.

YJ Wang, YL Guo, PF Cui, ZH Zhang, ZY Chen, H Lu… - 2020 - cabidigitallibrary.org
In order to analyze the expression and antiviral activity of recombinant duck interferon-γ
(DuIFNγ) in vivo and in vitro, the whole sequence encoded DuIFNγ published in GenBank …

Soluble expression of chicken interferon-gamma and antiviral activity of purified expression product

J Qi, Y Du, X Zhu, B Hu, S Sun, X Zhang… - Wei Sheng wu xue bao …, 2009 - europepmc.org
Objective We cloned and expressed chicken interferon-gamma gene (chIFN-gamma), and
detected the bioactivity of chIFN-gamma expressed in Escherichia coli (E. coli). Methods The …

Molecular cloning and sequencing analysis of the type I IFN from Beijing duck, anas platyrhynchos

X Chun, W Jianchun, W Zhiguang, W Ming… - Xu mu Shou yi xue bao …, 2000 - europepmc.org
In order to research the gene structure of interferon and lay a foundation for producing
recombinant Duck IFN as biological therapeutic agents, BDIFN-alpha gene was cloned from …

Prokaryotic expression and activity assay of recombinant chicken interferon-alpha.

JY Zheng, JH Chen, ZM Pan, XA Jiao - Zhongguo Yufang Shouyi …, 2009 - cabdirect.org
Chicken IFN-α gene was amplified by RT-PCR and cloned from chicken spleen lymphocytes
stimulated by concanavalin A. Recombinant plasmids (pGEX-proChlFN-α, pET-ChlFN-α) …

Expression of the mature protein gene of chicken γ-interferon and the detection for the antiviral activity of the purified expression product

CAI Mei-hong, CAO Rui-bing, Z Bin, TAN Guo-lei… - Virologica …, 2004 - virosin.org
Using the total mRNA in the lymphocyte in chicken blood as the template, the mature protein
gene of interferon γ-interferon was cloned and amplified through the reverse transcription …

Cloning, prokaryotic expression, and biological analysis of recombinant chicken IFN-γ

G Li, Y Zeng, J Yin, HS Lillehoj, X Ren - Hybridoma, 2010 - liebertpub.com
The full-length chicken interferon-gamma (CHIFN-γ) gene was amplified by reverse
transcription-PCR using total RNA extracted from the spleen cells of white Leghorn chicken …

Cloning, expression and antiviral activity of arctic fox (Alopex lagopus) interferon-gamma gene

H Zhang, X Chai, G Luo, F Wang, L Yi… - Sheng wu Gong …, 2008 - europepmc.org
In order to characterize the biological activity of fox (Vulpes vulpes) interferon gamma
(VuIFN-gamma), We have isolated the cDNA encoding arctic fox (Alopex lagopus) VuIFN …

Molecular cloning of swine interferon gamma cDNA and its expression in E. coli

W Wenxue, X Chun, W Ming, J Jinshu - Nong ye Sheng wu ji shu …, 2002 - europepmc.org
Swine interferon-gamma gene (SwIFN-# gamma# 2) was cloned from ConA-simulated
swine spleen cells total RNA by RT-PCR. SwIFN-# gamma# 2 was inserted into pGEM T …

High level expression of recombinant chicken interferon-gamma in insect cells

G Kong, J Xu, A Qin, W Jin… - Wei sheng wu xue bao …, 2005 - pubmed.ncbi.nlm.nih.gov
The recombinant transfer vector pFastBacl-ChIFN-y was constructed by plasmid pcDNA-
ChIFN-gamma digested with EcoR I and Not I enzymes and cloned into pFastbacl. Then the …