Gene manipulation is an important routine technique and its efficiency is often a rate-limiting
step in research. To facilitate gene manipulation in filamentous fungi, we adapted the S …

The rapid accumulation of genomic sequences from a large number of eukaryotes, including
numerous filamentous fungi, has created a tremendous scientific potential, which can only …

Advances in genetic transformation techniques have made important contributions to
molecular genetics. Various molecular tools and strategies have been developed for …

CONTENTS 30.1 Introduction.................................................................................................................
............ 493 30.2 General Considerations for Gene Manipulations …

A series of plasmid vectors, named pFungiway, was constructed for the purpose of simple
and versatile gene manipulation in a wide range of fungi. The backbone of these vectors …

The cloning of plasmids can be time-consuming or expensive. Yet, cloning is a prerequisite
for many standard experiments for the functional analysis of genes, including the generation …

Strategies for promoting high-efficiency homologous gene replacement have been
developed and adopted for many filamentous fungal species. The next generation of …

Technical advances in Aspergillus nidulans enable relatively easy deletion of genomic
sequences, insertion of sequences into the genome and alteration of genomic sequences …

A convenient method to remove selectable markers from fungal transformants permits the
markers to be used for sequential transformations, and should also reduce public concerns …

The construction of a fungal strain that lacks a specific gene product is often accomplished
by replacing the gene of interest with a selection marker using site-specific recombination …