Simultaneous improvements of Pseudomonas cell growth and polyhydroxyalkanoate production from a lignin derivative for lignin-consolidated bioprocessing

X Wang, L Lin, J Dong, J Ling, W Wang… - Applied and …, 2018 - Am Soc Microbiol
Cell growth and polyhydroxyalkanoate (PHA) biosynthesis are two key traits in PHA
production from lignin or its derivatives. However, the links between them remain poorly …

Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin

CW Johnson, GT Beckham - Metabolic engineering, 2015 - Elsevier
Lignin represents an untapped feedstock for the production of fuels and chemicals, but its
intrinsic heterogeneity makes lignin valorization a significant challenge. In nature, many …

[HTML][HTML] Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion

JR Elmore, GN Dexter, D Salvachúa… - Nature …, 2021 - nature.com
Expanding the portfolio of products that can be made from lignin will be critical to enabling a
viable bio-based economy. Here, we engineer Pseudomonas putida for high-yield …

[HTML][HTML] Adaptive laboratory evolution of Pseudomonas putida KT2440 improves p-coumaric and ferulic acid catabolism and tolerance

ET Mohamed, AZ Werner, D Salvachúa… - Metabolic engineering …, 2020 - Elsevier
Pseudomonas putida KT2440 is a promising bacterial chassis for the conversion of lignin-
derived aromatic compound mixtures to biofuels and bioproducts. Despite the inherent …

[HTML][HTML] Utilizing anaerobic fungi for two-stage sugar extraction and biofuel production from lignocellulosic biomass

A Ranganathan, OP Smith, NH Youssef… - Frontiers in …, 2017 - frontiersin.org
Lignocellulosic biomass is a vast and underutilized resource for the production of sugars
and biofuels. However, the structural complexity of lignocellulosic biomass and the need for …

[HTML][HTML] Engineering and Two-Stage Evolution of a Lignocellulosic Hydrolysate-Tolerant Saccharomyces cerevisiae Strain for Anaerobic Fermentation of Xylose from …

LS Parreiras, RJ Breuer, R Avanasi Narasimhan… - PloS one, 2014 - journals.plos.org
The inability of the yeast Saccharomyces cerevisiae to ferment xylose effectively under
anaerobic conditions is a major barrier to economical production of lignocellulosic biofuels …

Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate

E Kuatsjah, CW Johnson, D Salvachúa, AZ Werner… - Metabolic …, 2022 - Elsevier
Abstract The transformation of 4-hydroxybenzoate (4-HBA) to protocatechuate (PCA) is
catalyzed by flavoprotein oxygenases known as para-hydroxybenzoate-3-hydroxylases …

Investigation of the synergetic effect of xylose metabolic pathways on the production of glutaric acid

J Wang, X Shen, Y Lin, Z Chen, Y Yang… - ACS synthetic …, 2018 - ACS Publications
Efficient utilization of lignocellulose is pivotal for economically converting renewable
feedstocks into value-added products. Xylose is the second most abundant sugar in …

[HTML][HTML] Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae

X Qi, J Zha, GG Liu, W Zhang, BZ Li… - Frontiers in Microbiology, 2015 - frontiersin.org
Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising
approach to engineering heterologous pathway for xylose utilization in Saccharomyces …

[HTML][HTML] Reconstruction and optimization of a Pseudomonas putida-Escherichia coli microbial consortium for mcl-PHA production from lignocellulosic biomass

R Qin, Y Zhu, M Ai, X Jia - Frontiers in Bioengineering and …, 2022 - frontiersin.org
The demand for non-petroleum-based, especially biodegradable plastics has been on the
rise in the last decades. Medium-chain-length polyhydroxyalkanoate (mcl-PHA) is a …