Polyethylene glycol (PEG)-mediated transformation in filamentous fungal pathogens

Z Liu, TL Friesen - Plant fungal pathogens: Methods and protocols, 2012 - Springer
Genetic transformation is an essential tool for the modern study of gene function and the
genetic improvement of an organism. The genetic transformation of many fungal species is …

[HTML][HTML] Methods for genetic transformation of filamentous fungi

D Li, Y Tang, J Lin, W Cai - Microbial cell factories, 2017 - Springer
Filamentous fungi have been of great interest because of their excellent ability as cell
factories to manufacture useful products for human beings. The development of genetic …

Three decades of fungal transformation: key concepts and applications

V Olmedo-Monfil, C Cortés-Penagos… - … : Reviews and Protocols, 2004 - Springer
Filamentous fungi include a large, heterogeneous group of heterotrophic organisms with
profound influence in human activities. In spite of their economic and scientific relevance …

[HTML][HTML] Genetic transformation of the filamentous fungus Pseudogymnoascus verrucosus of Antarctic origin

A Díaz, P Villanueva, V Oliva, C Gil-Durán… - Frontiers in …, 2019 - frontiersin.org
Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus
Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive …

Strategies for the transformation of filamentous fungi

B Ruiz‐Díez - Journal of applied microbiology, 2002 - academic.oup.com
Molecular genetic manipulation of micro‐organisms requires the development of plasmid‐
mediated transformation systems that include:(i) infusion of exogenous DNA into recipient …

Agrobacterium-mediated transformation as a tool for functional genomics in fungi

CB Michielse, PJJ Hooykaas, CA van den Hondel… - Current genetics, 2005 - Springer
In the era of functional genomics, the need for tools to perform large-scale targeted and
random mutagenesis is increasing. A potential tool is Agrobacterium-mediated fungal …

Genetic transformation and vector developments in filamentous fungi

MBR Riach, JR Kinghorn - Fungal Genetics, 2020 - taylorfrancis.com
Recombinant DNA technology, which has provided techniques for the routine isolation and
analysis of genes from almost any organism, was originally successfully employed during …

PEG-mediated and Agrobacterium-mediated transformation in the mycopathogen Verticillium fungicola

A ATHEY-POLLARD, C BURNS, R Peter… - Mycological …, 2002 - cambridge.org
Verticillium fungicola, a severe mycopathogen of the cultivated mushroom Agaricus
bisporus, was successfully transformed using both PEG-mediated and Agrobacterium …

Agrobacterium tumefaciens-mediated transformation of filamentous fungi

MJA De Groot, P Bundock, PJJ Hooykaas… - Nature …, 1998 - nature.com
Agrobacterium tumefaciens transfers part of its Ti plasmid, the T-DNA, to plant cells during
tumorigenesis. It is routinely used for the genetic modification of a wide range of plant …

Alternative methods for genetic transformation of Pseudozyma antarctica, a basidiomycetous yeast-like fungus

G Marchand, E Fortier, B Neveu, S Bolduc… - Journal of …, 2007 - Elsevier
Electroporation and Agrobacterium tumefaciens-mediated transformation (ATMT) were
adapted and optimized for genetic transformation of the basidiomycetous yeast-like fungus …