The diagnosis of male infertility is vastly complex. To date, morphology, motility, and
concentration have been used as key parameters to establish the sperm normality and …

A full label-free morphological and biochemical characterization is desirable to select
spermatozoa during preparation for artificial insemination. In order to study these …

Raman microspectroscopy (RM) and polarization sensitive digital holographic imaging
(PSDHI) are valuable analytical tools in biological and medical research, allowing the …

Semen analysis is performed for evaluation of fertility disorders, however it is susceptible to
subjectivity of investigators, and lacking of objective criterion for sperm cell quality remains a …

Coupling digital holography with high-specific Raman spectroscopy is an attractive means of
identifying biochemical changes in cells by both physical topography and spectroscopy. We …

Growth in the percentage of spermatozoa with aberrant sperm head morphologies has been
correlated with a rise in male infertility. In our study, micro-Raman spectroscopy using a new …

Confocal Raman microspectroscopy was used to chemically image single, living and
immobilized human spermatozoa under near-physiological conditions. A novel combination …

Abstract STUDY QUESTION Is Raman micro-spectroscopy a valid approach to assess the
biochemical hallmarks of sperm regions (head, midpiece and tail) in four different species …

Confocal Raman microspectroscopy was used to investigate human sperm cells. Raman
mapping with a 532 nm excitation laser allowed to unambiguously characterize the nucleus …

Chromatin condensation is one of the main factors essential for sperm function. Evaluation
of chromatin condensation by current methods render the assessed sperm unsuitable for …