A systematic evaluation of single cell RNA-seq analysis pipelines
The recent rapid spread of single cell RNA sequencing (scRNA-seq) methods has created a
large variety of experimental and computational pipelines for which best practices have not …
large variety of experimental and computational pipelines for which best practices have not …
Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …
you to profile the whole transcriptome of a large number of individual cells. However, the …
Over 1000 tools reveal trends in the single-cell RNA-seq analysis landscape
Recent years have seen a revolution in single-cell RNA-sequencing (scRNA-seq)
technologies, datasets, and analysis methods. Since 2016, the scRNA-tools database has …
technologies, datasets, and analysis methods. Since 2016, the scRNA-tools database has …
A statistical simulator scDesign for rational scRNA-seq experimental design
Motivation Single-cell RNA sequencing (scRNA-seq) has revolutionized biological sciences
by revealing genome-wide gene expression levels within individual cells. However, a critical …
by revealing genome-wide gene expression levels within individual cells. However, a critical …
Comparative analysis of sequencing technologies for single-cell transcriptomics
Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we
present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq …
present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq …
[HTML][HTML] Single-cell RNA-seq technologies and related computational data analysis
G Chen, B Ning, T Shi - Frontiers in genetics, 2019 - frontiersin.org
Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene
expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A …
expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A …
Introduction to single‐cell RNA sequencing
TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …
Single-cell RNA sequencing analysis: a step-by-step overview
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …
individual cells can now be measured for thousands of cells in a single experiment. Since its …
Exploring the single-cell RNA-seq analysis landscape with the scRNA-tools database
As single-cell RNA-sequencing (scRNA-seq) datasets have become more widespread the
number of tools designed to analyse these data has dramatically increased. Navigating the …
number of tools designed to analyse these data has dramatically increased. Navigating the …
[HTML][HTML] Reproducibility of methods to detect differentially expressed genes from single-cell RNA sequencing
Detection of differentially expressed genes is a common task in single-cell RNA-seq (scRNA-
seq) studies. Various methods based on both bulk-cell and single-cell approaches are in …
seq) studies. Various methods based on both bulk-cell and single-cell approaches are in …