… To validate the accuracy of the splice isoforms detected with SMRT, we randomly selected
5 … size of the fragments were consistent with the splice isoforms identified from Iso-Seq data, …

… splicing pattern as predicted in the reference genome. Isoforms belong to NNIC, isoforms with
novel splice … , demonstrating the usefulness of Iso-Seq to discover new isoforms in alfalfa. …

… Relative to the predominant transcript isoform, AS can be divided into four main types,
intron … sativa) and clover (Trifolium sp.) as well as the model plant M. truncatula. Medicago …

… Contigs expected to represent splice variants of a single gene were merged into so-called
“supertranscripts” using the SuperTranscripts program, resulting in compressed assemblies of …

… the proteome-level changes associated with salt stress response in Medicago sativa cv.
Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. Our physiological and phenotypic …

… splicing at the … splicing variants were sensitive to nonsense-mediated decay, the premature
termination codon-harboring alternative splicing variants sharing the 3′ alternative splicing …

… of the gene encoding calmodulin isoform 4 (GmCaM4) and are … Alfalfa (Medicago sativa L.)
is the major forage protein … 1 were identified (only the longest splice variant was reserved at …

… To verify the PacBio isoforms and remove isoforms with false splice junctions, we analysed
… Second, taking a comprehensive view, we first identified splice variants at the whole-genome …

… Alfalfa (Medicago sativa L.) is a major perennial forage legume crop with numerous … For
CAD which had two splice forms, 30 and 31 variants (out of 1077 and 906 base pairs of the …

… Freezing stress is a major limiting environmental factor that affects the productivity and
distribution of alfalfa (Medicago sativa L.). There is growing evidence that enhancing freezing …