[HTML][HTML] A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos

AF Ermisch, JR Herrick, R Pasquariello, MKC Dyer… - Scientific reports, 2020 - nature.com
… than what is supplied in traditional culture media. Our objective was to determine the
impact of reduced nutrient concentrations in culture medium on mouse embryo development, …

Culture of the mouse preimplantation embryo

DK Gardner, TT Truong - … Embryo Culture: Methods and Protocols, 2019 - Springer
… tract, culture media to support mouse embryo development in … Metabolite concentrations
in culture media have received … disturbance of the embryo in vitro, reducing both temperature …

β-hydroxybutyrate reduces blastocyst viability via trophectoderm-mediated metabolic aberrations in mice

EG Whatley, TT Truong, D Wilhelm… - Human …, 2022 - academic.oup.com
… and used in the in vitro embryo culture medium in this study (in … preimplantation embryos
can metabolize βOHB, a novel … Indeed, this was supported by embryo transfer experiments in …

Antioxidant supplementation of mouse embryo culture or vitrification media support more in-vivo-like gene expression post-transfer

T Truong, AJ Harvey, DK Gardner - Reproductive BioMedicine Online, 2022 - Elsevier
… in-vitro culture media significantly reduced the expression of … GSH concentrations to those
comparable to in-vivo-derived … are not limited to metabolic function and nutrient transport, cell …

Making and selecting the best embryo in the laboratory

DK Gardner, D Sakkas - Fertility and sterility, 2023 - Elsevier
… to select the most viable embryo for transfer reduces both time to … of media supported cleavage
stage embryo development, … gone into the formulations of culture media over the past 40 …

[HTML][HTML] Hypoxic in vitro culture reduces histone lactylation and impairs pre-implantation embryonic development in mice

W Yang, P Wang, P Cao, S Wang, Y Yang, H Su… - Epigenetics & …, 2021 - Springer
… 6B, C), supporting the hypothesis that histone lactylation is … and the composition of amino
acids in the culture medium [52… a novel example of metabolic–epigenetic interplay in mouse pre…

[HTML][HTML] Selected amino acids promote mouse pre-implantation embryo development in a growth factor-like manner

MB Morris, S Ozsoy, M Zada, M Zada… - Frontiers in …, 2020 - frontiersin.org
… , mouse embryos provide autocrine/paracrine support for … We note that mTOR –/– mouse
embryos exhibit reduced … rational design of improved culture media for embryos depends on …

Embryo responses to stress induced by assisted reproductive technologies

P Ramos‐Ibeas, S Heras… - … and development, 2019 - Wiley Online Library
… of culturing mouse embryos under low oxygen tension (3% … culture medium to produce
more in vivo-like embryos. Many supplements have been tested to support embryo development. …

Reducing time to pregnancy and facilitating the birth of healthy children through functional analysis of embryo physiology

L Ferrick, YSL Lee, DK Gardner - Biology of Reproduction, 2019 - academic.oup.com
embryo to support viable development. Potential protein targets identified within preimplantation
embryo spent culture media, ie, the embryonic … However, with the development of novel

[HTML][HTML] An improved method of maintaining primary murine cardiac fibroblasts in two-dimensional cell culture

NM Landry, SG Rattan, IMC Dixon - Scientific reports, 2019 - nature.com
… You are using a browser version with limited support for CSS. … limiting the nutrient content
of cell culture media can extend … In the heart, fibroblasts are derived from different embryonic