Atomic-resolution mapping of transcription factor-DNA interactions by femtosecond laser crosslinking and mass spectrometry

A Reim, R Ackermann, J Font-Mateu, R Kammel… - Nature …, 2020 - nature.com
… As the pulse length is determined by the laser setup, we … we could narrow down the
cross-link localization to stretches of … , which in a previous mutation study conferred DNA-binding …

Electron and X-ray focused beam-induced cross-linking in liquids: toward rapid continuous 3D Nanoprinting and interfacing using soft materials

T Gupta, E Strelcov, G Holland, J Schumacher… - ACS …, 2020 - ACS Publications
… In this article, we demonstrate that polymerization and cross-link … the liquid sample chamber
used in this study. (d) 3D surface plot … 150 nm in our setups. On the other hand, the effective …

High-density chemical cross-linking for modeling protein interactions

J Mintseris, SP Gygi - … of the National Academy of Sciences, 2020 - National Acad Sciences
… methods to obtain density of 1 cross-link per 7 amino acids of protein … –Lys experiment by
50%, with BS3 spanning a longer cross-link … Cytoscape’s (44) “organic” layout algorithm readily …

LinX: a software tool for uncommon cross-linking chemistry

Z Kukačka, M Rosůlek, J Jelínek… - Journal of Proteome …, 2021 - ACS Publications
… The experimental setup of protein cross-linking procedures followed … –protein cross-link
(blue) compared with experimental data (red). (B) Example of the protein–nucleic acid cross-link

Cross-linking mass spectrometry for investigating protein conformations and protein–protein interactions─ a method for all seasons

L Piersimoni, PL Kastritis, C Arlt, A Sinz - Chemical Reviews, 2021 - ACS Publications
… It monitors the rates of exchanging hydrogen by deuterium atoms at the amide backbone …
study performed using PIRs. In this study, isotope-labeled PIRs were used to in vivo cross-link

Cross-linking of polymers by various radiations: Mechanisms and parameters

ST Mhaske, SU Mestry, DA Patil - Radiation Technologies and …, 2022 - taylorfrancis.com
… that the flexible chains are more accessible to cross-link than are rigid chains (Jiazhen, 2001…
effect of the quaternary carbon atom. A polyacrylate is easy to cross-link by radiation, while …

The conformational preference of chemical cross-linkers determines the cross-linking probability of reactive protein residues

Z Gong, SX Ye, ZF Nie, C Tang - The Journal of Physical …, 2020 - ACS Publications
… the distribution profile of the cross-link distances is determined by … XLMS data, we analyzed
the experimental cross-links. We show … Using the parameters from QM, we set up the all-atom

[HTML][HTML] Illustration of distinct nuclear radiation transmission factors combined with physical and elastic characteristics of barium boro-bismuthate glasses

G Lakshminarayana, A Kumar, HO Tekin, SAM Issa… - Results in Physics, 2021 - Elsevier
For B 2 O 3 -Bi 2 O 3 -BaO glass system, γ, proton, alpha, electron, and neutron radiation
shielding aspects were examined. Adopting Phy-X/PSD software, mass attenuation coefficients …

NMR‐Based CrossLink Densities in EPDM and EPDM/ULDPE Blend Materials and Correlation with Mechanical Properties

A Karekar, R Pommer, B Prem… - Macromolecular …, 2022 - Wiley Online Library
Cross-link density in EPDM using different cross-linker systems has already been studied …
polarization-MAS experiments. [ 34 ] In a time-domain study, cross-link densities have been …

Tightening the crosslinking distance restraints for better resolution of protein structure and dynamics

Z Gong, SX Ye, C Tang - Structure, 2020 - cell.com
… for Cα-Cα atoms of pairwise lysine residues involved in experimental intramolecular BS 3 …
group is planar in a trans configuration for the nitrogen and oxygen atoms (Figure 2C). The …