… The factors influencing the low expression of ectC were not yet understood. The aligned
spacing between the Shine-Dalgarno (SD) sequence and the start codon AUG played an …

… Although the specific codon optimization methods applied in this study were not the main
focus, some conclusions can be drawn regarding these methods. First, there is no algorithm …

… have been successfully obtained through recombinant production in Escherichia coli [5,6], …
The optimized procedure allows the purification of about 10–15 mg of pure peptide from 1 L …

… extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the diversity
of Escherichia … We then trace the history of cell-extract methodology over the past 20 years, …

… Using this method, we have generated a set of 12 optimized sensors with high dynamic range
and low cross-reactivity. These have been integrated into the genomes of E. coli MG1655 (…

… One of the main factors affecting metabolic burden is promoter strength. lac-derived
promoters (P T7 lac , P trc , and P tac ) are all based on the negative regulation by LacI, and the …

… The resulting ec_iML1515 model showed that the protein demand and protein synthesis
rate were the key factors affecting lysine production. By optimizing the expression of the 20 top-…

… planktonic and biofilm cells of Escherichia coli JM109(DE3) transformed with a plasmid
containing a T7 promoter. It was shown that induction negatively affected the growth and viability …

… , including substrate and product toxicity, formation of undesired products and genetic
stability of the recombinant strains, that impede an efficient use of recombinant Escherichia coli …

… exonuclease and then transformed into Escherichia coli to produce clone colonies. The
cloning efficiency was similar to that of the commercial In-Fusion method employing a proprietary …