Lasy-Seq: a high-throughput library preparation method for RNA-Seq and its application in the analysis of plant responses to fluctuating temperatures

M Kamitani, M Kashima, A Tezuka, AJ Nagano - Scientific Reports, 2019 - nature.com
… established a high-throughput and cost-effective RNA-Seq library preparation method
that … described in this study, we recommend the use of the Illumina platform with non-patterned …

Systematic evaluation of RNA-Seq preparation protocol performance

HP Chao, Y Chen, Y Takata, MW Tomida, K Lin, JS Kirk… - BMC genomics, 2019 - Springer
… input protocols: Illumina TruSeq … methods to understand the strength of each protocol. The
first goal of our study was to investigate confounding factors in RNA-Seq library preparation

Bias in RNAseq library preparation: current challenges and solutions

H Shi, Y Zhou, E Jia, M Pan, Y Bai… - BioMed research …, 2021 - Wiley Online Library
… sequencing, high-throughput sequencing (NGS) of RNA (RNA-seq) … However, RNA-seq is
a process of extremely intricate, … [39] proposed an alternative to RNA-seq using the Illumina

A comparison between whole transcript and 3'RNA sequencing methods using Kapa and Lexogen library preparation methods

F Ma, BK Fuqua, Y Hasin, C Yukhtman, CD Vulpe… - BMC genomics, 2019 - Springer
High-throughput RNA-sequencing (RNA-Seq) is a powerful … library preparation, we pooled
and sequenced the libraries … reads on an Illumina HiSeq4000 instrument (Illumina, San Diego…

Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing

L Zhou, HK Ng, DI Drautz-Moses, SC Schuster… - Scientific reports, 2019 - nature.com
… Having systematically evaluated the performance of the three library preparation methods,
we next compared performance between the two leading Illumina high-throughput

Miniaturization and optimization of 384-well compatible RNA sequencing library preparation

MY Mayday, LM Khan, ED Chow, MS Zinter, JL DeRisi - PloS one, 2019 - journals.plos.org
Illumina sequencing platforms, to miniaturize and automate mNGS … library preparation
techniques, we sought to adapt our mNGS library preparation protocol into a high-throughput

Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems

X Zhang, T Li, F Liu, Y Chen, J Yao, Z Li, Y Huang… - Molecular cell, 2019 - cell.com
… , single-cell RNA sequencing (RNA-seq) has been a major … of ultra-high-throughput
single-cell RNA-seq has demonstrated … ultra-high-throughput single-cell RNA-seq systems have …

BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

D Alpern, V Gardeux, J Russeil, B Mangeat… - Genome biology, 2019 - Springer
… All RNA-seq library preparation methods are globally relying on the … RNA-seq library
preparation workflow First, we set out to benchmark SCRB-seq against the “gold standard” Illumina

An RNA-Seq protocol for differential expression analysis

NDL Owens, E De Domenico… - Cold Spring Harbor …, 2019 - cshprotocols.cshlp.org
… the power of high-throughput sequencing to understand … and treated embryos) using Illumina
sequencing. Samples contain … low-RNA samples during library preparation as this can lead …

Microfluidic platform for Next-Generation Sequencing library preparation with low-input samples

TW Murphy, YP Hsieh, B Zhu, LB Naler… - Analytical chemistry, 2020 - ACS Publications
library preparation system for high-throughput and low-input processing starting with
primary tissues, we prepared 8 libraries … applicable to all Illumina-based library preparation. …