[图书][B] Gene cloning and DNA analysis: an introduction

TA Brown - 2020 - books.google.com
… continuing evolution of next‐generation DNA sequencing is … Usually only one recombinant
DNA molecule is transported … may contain many different recombinant DNA molecules, each …

[PDF][PDF] Useful vectors for the two-hybrid system in mammalian cells

M Buchert, S Schneider, MT Adams, HP Hefti… - …, 2024 - Taylor & Francis
… from any recombinants tested. We have therefore tagged the GAL4p … and sequences of the
inserted synthetic cassettes by DNARestriction enzyme sites that occur within the bacterial …

[图书][B] Molecular biotechnology: principles and applications of recombinant DNA

BR Glick, CL Patten - 2022 - books.google.com
restriction endonuclease and mixed together, new DNA … and DNA ligase to generate
hybrid DNA molecules.” The … to the development of recombinant DNA technology because it …

Genomic profiling of native R loops with a DNA-RNA hybrid recognition sensor

K Wang, H Wang, C Li, Z Yin, R Xiao, Q Li, Y Xiang… - Science …, 2021 - science.org
… ), and DNA-RNA hybrid, we tested the affinity of recombinant GST-His 6 … and subsequent
next-generation sequencing. The … with the bias of fragmentation by restriction enzymes (21), we …

DNA-RNA hybrids at DSBs interfere with repair by homologous recombination

P Ortega, JA Mérida-Cerro, AG Rondón… - Elife, 2021 - elifesciences.org
… at each of the FRT sites would lead to Lys+ recombinants by allelic recombination between
… On the one hand, a cohort of factors has evolved to prevent or remove DNA-RNA hybrids co-…

Directed co-evolution of interacting protein–peptide pairs by compartmentalized two-hybrid replication (C2HR)

JW Siau, S Nonis, S Chee, LQ Koh… - Nucleic Acids …, 2020 - academic.oup.com
… from Integrated DNA Technologies; restriction enzymes, T4 … sequencing carried out using
the NextSeq Illumina platform (… , and titrate levels of recombinant Sso7d–SpyCatcher adding …

Role of recombinant DNA technology in agriculture.

T Begna, JC Okonkwo - 2020 - cabidigitallibrary.org
… to create and study these hybrid molecules. The power of … the recombinant DNA (depending
upon the restriction enzyme … follicular development is an achievement of recombinant DNA

[HTML][HTML] Mitochondrial DNA duplication, recombination, and introgression during interspecific hybridization

S Bágeľová Poláková, Ž Lichtner, T Szemes… - Scientific Reports, 2021 - nature.com
… that recombinant mtDNA molecules were not observed in early interspecific hybrids. …
Restriction endonuclease analysis of mtDNA is widely used to distinguish between different yeast …

Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo

H Park, S Kim - Nucleic Acids Research, 2021 - academic.oup.com
DNA polymerase (Toyobo, Japan). Restriction enzymes, T4 polynucleotide kinase and T4
DNAsequenced ∼3.3 kb DNA around the target gene from cells taken at cycle 27 by Illumina …

[HTML][HTML] Improving selectivity of DNA–RNA binding zinc finger using directed evolution

AA Sulej - BMC research notes, 2019 - Springer
sequences in DNA–RNA hybrids using directed evolution of … to how restriction enzymes
are applied for dsDNA cleavage. … mixture of T7 phages with recombinant T7ZfQQR in a 100:1 …