Droplet-based microfluidic platform for high-throughput screening of Streptomyces

R Tu, Y Zhang, E Hua, L Bai, H Huang, K Yun… - Communications …, 2021 - nature.com
library of the SLATG and SLACG null strains, with the SLATG … were sorted and cultivated for
sequencing verification (Fig. 4a). … , re-cultivated, and sequenced for their mutated regions. To …

A genetics-free method for high-throughput discovery of cryptic microbial metabolites

F Xu, Y Wu, C Zhang, KM Davis, K Moon… - Nature chemical …, 2019 - nature.com
… We report a universal high-throughput method for activating … metabolome of a given strain
and imaging mass spectrometry to … To verify these results, we collected high-resolution NOESY …

High-throughput discovery of phage receptors using transposon insertion sequencing of bacteria

KE Kortright, BK Chan… - Proceedings of the …, 2020 - National Acad Sciences
… on very large numbers of mutant bacterial strains if genes for … We further verified these
conclusions using classic adsorption (… DNA libraries were pooled and sequenced at the Yale …

Highthroughput metagenomics for identification of pathogens in the clinical settings

N Li, Q Cai, Q Miao, Z Song, Y Fang, B Hu - Small methods, 2021 - Wiley Online Library
… Oxford Nanopore DNA library construction methods can be … is greater than that between
strains of the same species when … The virus genomic sequences are checked and verified

High-throughput microbial culturomics using automation and machine learning

Y Huang, RU Sheth, S Zhao, LA Cohen… - Nature …, 2023 - nature.com
… Here we describe an open-source high-throughput robotic strain isolation platform for the
rapid generation of isolates on demand. We develop a machine learning approach that …

High-throughput mapping of the phage resistance landscape in E. coli

VK Mutalik, BA Adler, HS Rishi, D Piya, C Zhong… - PLoS …, 2020 - journals.plos.org
… coli K-12 strain and 14 diverse double-stranded DNA (dsDNA… pathways, and they also
yield additional novel loci that … the DNA barcode associated with each strain. The individual …

Fine‐tuning the (2S)‐naringenin synthetic pathway using an iterative highthroughput balancing strategy

S Zhou, Y Lyu, H Li, MAG Koffas… - Biotechnology and …, 2019 - Wiley Online Library
… The metabolic flux of the screened high-titer strains was … verify this speculation, the upstream
promoter of CHS in 9G3 strain was replaced by a T7 promoter and resulted in a strain of E. …

Reduced fire blight susceptibility in apple cultivars using a highefficiency CRISPR/Cas9‐FLP/FRT‐based gene editing system

V Pompili, L Dalla Costa, S Piazza… - Plant biotechnology …, 2020 - Wiley Online Library
… exogenous DNA elimination. The T-DNA removal was further validated by sequencing in …
plants inoculated by the method of scissor with Erwinia amylovora strain Ea273. The number of …

Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale

FE Liew, R Nogle, T Abdalla, BJ Rasor, C Canter… - Nature …, 2022 - nature.com
library approach, we first mined a historical industrial strain … -up our optimized strains for
continuous production at rates of … combinatorial plasmids were sequence verified using MiSeq …

Synthetic community with six Pseudomonas strains screened from garlic rhizosphere microbiome promotes plant growth

L Zhuang, Y Li, Z Wang, Y Yu, N Zhang… - Microbial …, 2021 - Wiley Online Library
… After analysis based on Illumina-sequenced database and sequence alignment of isolated
bacteria, six Pseudomonas sp. strains with different phylogenetic names and B8-7 strain